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XB-ART-54226
Genesis January 1, 2017; 55 (12):

Znf703, a novel target of Pax3 and Zic1, regulates hindbrain and neural crest development in Xenopus.



Abstract
The transcription factors Pax3 and Zic1 are critical to specify the neural plate border and to promote neural crest formation. In a microarray screen designed to identify genes regulated by Pax3 and Zic1 in Xenopus we isolated Znf703/Nlz1 a transcriptional repressor member of the NET (NocA/Nlz, Elbow, and TLP-1) protein family. At early neurula stage znf703 is expressed in the dorsal ectoderm, spanning the neural plate and neural plate border, with an anterior boundary of expression corresponding to rhombomeres 3 and 4 (r3/r4) in the prospective hindbrain. As a bonafide target of Pax3 and Zic1, znf703 is activated by neural plate border inducing signals, and its expression depends on Pax3 and Zic1 function in the embryo. Znf703 morpholino-mediated knockdown expanded several posterior hindbrain genes, while Znf703 overexpression completely obliterated the expression of these segmental genes, signifying that the transcriptional repressor activity of Znf703 is critical to pattern the hindbrain. Furthermore, snai2 and sox10 expression was severely impaired upon manipulation of Znf703 expression levels in the embryo suggesting that Znf703 participates in neural crest formation downstream of Pax3 and Zic1 in Xenopus.

PubMed ID: 29086464
PMC ID: PMC5734999
Article link: Genesis
Grant support: [+]
Genes referenced: egr2 egr4 fgf3 hnf1b hoxa2 hoxb1 mafb nog pax3 snai2 sox1 sox10 sox2 wnt1 zic1 znf703
GO keywords: neural crest cell development [+]
Morpholinos: pax3 MO1 zic1 MO1 znf703 MO3


Article Images: [+] show captions
References [+] :
Aoki, Sox10 regulates the development of neural crest-derived melanocytes in Xenopus. 2003, Pubmed, Xenbase


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