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Genesis December 1, 2017; 55 (12):

Znf703, a novel target of Pax3 and Zic1, regulates hindbrain and neural crest development in Xenopus.

The transcription factors Pax3 and Zic1 are critical to specify the neural plate border and to promote neural crest formation. In a microarray screen designed to identify genes regulated by Pax3 and Zic1 in Xenopus we isolated Znf703/Nlz1 a transcriptional repressor member of the NET (NocA/Nlz, Elbow, and TLP-1) protein family. At early neurula stage znf703 is expressed in the dorsal ectoderm, spanning the neural plate and neural plate border, with an anterior boundary of expression corresponding to rhombomeres 3 and 4 (r3/r4) in the prospective hindbrain. As a bonafide target of Pax3 and Zic1, znf703 is activated by neural plate border inducing signals, and its expression depends on Pax3 and Zic1 function in the embryo. Znf703 morpholino-mediated knockdown expanded several posterior hindbrain genes, while Znf703 overexpression completely obliterated the expression of these segmental genes, signifying that the transcriptional repressor activity of Znf703 is critical to pattern the hindbrain. Furthermore, snai2 and sox10 expression was severely impaired upon manipulation of Znf703 expression levels in the embryo suggesting that Znf703 participates in neural crest formation downstream of Pax3 and Zic1 in Xenopus.

PubMed ID: 29086464
PMC ID: PMC5734999
Article link: Genesis
Grant support: R01 DE014212 NIDCR NIH HHS, R01 DE025468 NIDCR NIH HHS

Genes referenced: egr2 egr4 fgf3 hnf1b hoxa2 hoxb1 mafb pax3 zic1 znf703 snai2 sox2 sox1

Morpholinos referenced: pax3 MO1 zic1 MO1 znf703 MO3

External Resources: GO Terms referenced: neural crest cell development [+]

Article Images: [+] show captions

Aoki, 2003, Pubmed, Xenbase [+]

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