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Prog Mol Subcell Biol January 1, 2017; 56 59-84.

The Power of Xenopus Egg Extract for Reconstitution of Centromere and Kinetochore Function.

Faithful transmission of genetic information during cell division requires attachment of chromosomes to the mitotic spindle via the kinetochore. In vitro reconstitution studies are beginning to uncover how the kinetochore is assembled upon the underlying centromere, how the kinetochore couples chromosome movement to microtubule dynamics, and how cells ensure the site of kinetochore assembly is maintained from one generation to the next. Here we give special emphasis to advances made in Xenopus egg extract, which provides a unique, biochemically tractable in vitro system that affords the complexity of cytoplasm and nucleoplasm to permit reconstitution of the dynamic, cell cycle-regulated functions of the centromere and kinetochore.

PubMed ID: 28840233
PMC ID: PMC5712478
Article link: Prog Mol Subcell Biol
Grant support: [+]

References [+] :
Abrieu, Mps1 is a kinetochore-associated kinase essential for the vertebrate mitotic checkpoint. 2001, Pubmed, Xenbase

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