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Cell Mol Life Sci
2018 Mar 01;756:1133-1144. doi: 10.1007/s00018-017-2716-5.
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Structural variations in wheat HKT1;5 underpin differences in Na+ transport capacity.
Xu B
,
Waters S
,
Byrt CS
,
Plett D
,
Tyerman SD
,
Tester M
,
Munns R
,
Hrmova M
,
Gilliham M
.
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An important trait associated with the salt tolerance of wheat is the exclusion of sodium ions (Na+) from the shoot. We have previously shown that the sodium transporters TmHKT1;5-A and TaHKT1;5-D, from Triticum monoccocum (Tm) and Triticum aestivum (Ta), are encoded by genes underlying the major shoot Na+-exclusion loci Nax1 and Kna1, respectively. Here, using heterologous expression, we show that the affinity (K m) for the Na+ transport of TmHKT1;5-A, at 2.66 mM, is higher than that of TaHKT1;5-D at 7.50 mM. Through 3D structural modelling, we identify residues D471/a gap and D474/G473 that contribute to this property. We identify four additional mutations in amino acid residues that inhibit the transport activity of TmHKT1;5-A, which are predicted to be the result of an occlusion of the pore. We propose that the underlying transport properties of TmHKT1;5-A and TaHKT1;5-D contribute to their unique ability to improve Na+ exclusion in wheat that leads to an improved salinity tolerance in the field.
CE1400008 Centre of Excellence in Plant Energy Biology, Australian Research Council, FT130100709 ARC Future Fellowship, DP120100900 ARC Discorvery, DE150100837 DECRA
Ali,
A Single Amino-Acid Substitution in the Sodium Transporter HKT1 Associated with Plant Salt Tolerance.
2016, Pubmed,
Xenbase
Ali,
A Single Amino-Acid Substitution in the Sodium Transporter HKT1 Associated with Plant Salt Tolerance.
2016,
Pubmed
,
Xenbase
Almeida,
Differences in shoot Na+ accumulation between two tomato species are due to differences in ion affinity of HKT1;2.
2014,
Pubmed
,
Xenbase
Almeida,
HKT transporters--state of the art.
2013,
Pubmed
Ariyarathna,
Characterization of the multigene family TaHKT 2;1 in bread wheat and the role of gene members in plant Na(+) and K(+) status.
2014,
Pubmed
Asins,
Two closely linked tomato HKT coding genes are positional candidates for the major tomato QTL involved in Na+ /K+ homeostasis.
2013,
Pubmed
Baxter,
A coastal cline in sodium accumulation in Arabidopsis thaliana is driven by natural variation of the sodium transporter AtHKT1;1.
2010,
Pubmed
Ben Amar,
Functional characterization in Xenopus oocytes of Na+ transport systems from durum wheat reveals diversity among two HKT1;4 transporters.
2014,
Pubmed
,
Xenbase
Byrt,
The Na(+) transporter, TaHKT1;5-D, limits shoot Na(+) accumulation in bread wheat.
2014,
Pubmed
,
Xenbase
Byrt,
HKT1;5-like cation transporters linked to Na+ exclusion loci in wheat, Nax2 and Kna1.
2007,
Pubmed
Böhm,
Venus Flytrap HKT1-Type Channel Provides for Prey Sodium Uptake into Carnivorous Plant Without Conflicting with Electrical Excitability.
2016,
Pubmed
,
Xenbase
Cao,
Crystal structure of a potassium ion transporter, TrkH.
2011,
Pubmed
Corratgé-Faillie,
Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family.
2010,
Pubmed
Cotsaftis,
A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing.
2012,
Pubmed
Diatloff,
Site directed mutagenesis reduces the Na+ affinity of HKT1, an Na+ energized high affinity K+ transporter.
1998,
Pubmed
Edgar,
MUSCLE: multiple sequence alignment with high accuracy and high throughput.
2004,
Pubmed
Fairbairn,
Characterisation of two distinct HKT1-like potassium transporters from Eucalyptus camaldulensis.
2000,
Pubmed
,
Xenbase
Gierth,
Potassium transporters in plants--involvement in K+ acquisition, redistribution and homeostasis.
2007,
Pubmed
Gille,
Sequence alignment visualization in HTML5 without Java.
2014,
Pubmed
Horie,
HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants.
2009,
Pubmed
Huang,
Crystal structure of the potassium-importing KdpFABC membrane complex.
2017,
Pubmed
Ismail,
Genomics, Physiology, and Molecular Breeding Approaches for Improving Salt Tolerance.
2017,
Pubmed
Jabnoune,
Diversity in expression patterns and functional properties in the rice HKT transporter family.
2009,
Pubmed
,
Xenbase
James,
Physiological characterization of two genes for Na+ exclusion in durum wheat, Nax1 and Nax2.
2006,
Pubmed
James,
Major genes for Na+ exclusion, Nax1 and Nax2 (wheat HKT1;4 and HKT1;5), decrease Na+ accumulation in bread wheat leaves under saline and waterlogged conditions.
2011,
Pubmed
Kelley,
Protein structure prediction on the Web: a case study using the Phyre server.
2009,
Pubmed
Kumar,
Physiological, Biochemical, Epigenetic and Molecular Analyses of Wheat (Triticum aestivum) Genotypes with Contrasting Salt Tolerance.
2017,
Pubmed
Landau,
ConSurf 2005: the projection of evolutionary conservation scores of residues on protein structures.
2005,
Pubmed
Laurie,
A role for HKT1 in sodium uptake by wheat roots.
2002,
Pubmed
Liu,
Characterization of two HKT1 homologues from Eucalyptus camaldulensis that display intrinsic osmosensing capability.
2001,
Pubmed
,
Xenbase
Läuchli,
Cell-specific localization of Na+ in roots of durum wheat and possible control points for salt exclusion.
2008,
Pubmed
Mian,
Over-expression of an Na+-and K+-permeable HKT transporter in barley improves salt tolerance.
2011,
Pubmed
,
Xenbase
Mishra,
Association of SNP Haplotypes of HKT Family Genes with Salt Tolerance in Indian Wild Rice Germplasm.
2016,
Pubmed
Munns,
Wheat grain yield on saline soils is improved by an ancestral Na⁺ transporter gene.
2012,
Pubmed
,
Xenbase
Munns,
Salinity tolerance of crops - what is the cost?
2015,
Pubmed
Mäser,
Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants.
2002,
Pubmed
,
Xenbase
Møller,
Shoot Na+ exclusion and increased salinity tolerance engineered by cell type-specific alteration of Na+ transport in Arabidopsis.
2009,
Pubmed
Negrão,
New allelic variants found in key rice salt-tolerance genes: an association study.
2013,
Pubmed
Pei,
PROMALS3D: a tool for multiple protein sequence and structure alignments.
2008,
Pubmed
Platten,
Nomenclature for HKT transporters, key determinants of plant salinity tolerance.
2006,
Pubmed
Plett,
Improved salinity tolerance of rice through cell type-specific expression of AtHKT1;1.
2010,
Pubmed
Ren,
A rice quantitative trait locus for salt tolerance encodes a sodium transporter.
2005,
Pubmed
Rodríguez-Navarro,
Dual system for potassium transport in Saccharomyces cerevisiae.
1984,
Pubmed
Rubio,
Sodium-driven potassium uptake by the plant potassium transporter HKT1 and mutations conferring salt tolerance.
1995,
Pubmed
,
Xenbase
Sali,
Comparative protein modelling by satisfaction of spatial restraints.
1993,
Pubmed
Schachtman,
Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants.
1994,
Pubmed
,
Xenbase
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Shen,
Statistical potential for assessment and prediction of protein structures.
2006,
Pubmed
Singh,
Induced defence responses of contrasting bread wheat genotypes under differential salt stress imposition.
2015,
Pubmed
Sippl,
Recognition of errors in three-dimensional structures of proteins.
1993,
Pubmed
Tounsi,
Characterization of Two HKT1;4 Transporters from Triticum monococcum to Elucidate the Determinants of the Wheat Salt Tolerance Nax1 QTL.
2016,
Pubmed
,
Xenbase
Uozumi,
The Arabidopsis HKT1 gene homolog mediates inward Na(+) currents in xenopus laevis oocytes and Na(+) uptake in Saccharomyces cerevisiae.
2000,
Pubmed
,
Xenbase
Vieira-Pires,
The structure of the KtrAB potassium transporter.
2013,
Pubmed
Waters,
Plant High-Affinity Potassium (HKT) Transporters involved in salinity tolerance: structural insights to probe differences in ion selectivity.
2013,
Pubmed
Wu,
LOMETS: a local meta-threading-server for protein structure prediction.
2007,
Pubmed
Zhang,
I-TASSER server for protein 3D structure prediction.
2008,
Pubmed
