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XB-ART-54451
Proc Natl Acad Sci U S A January 1, 2018; 115 (4): 726-731.

Functional characterization of the mucus barrier on the Xenopus tropicalis skin surface.

Dubaissi E , Rousseau K , Hughes GW , Ridley C , Grencis RK , Roberts IS , Thornton DJ .


Abstract
Mucosal surfaces represent critical routes for entry and exit of pathogens. As such, animals have evolved strategies to combat infection at these sites, in particular the production of mucus to prevent attachment and to promote subsequent movement of the mucus/microbe away from the underlying epithelial surface. Using biochemical, biophysical, and infection studies, we have investigated the host protective properties of the skin mucus barrier of the Xenopus tropicalis tadpole. Specifically, we have characterized the major structural component of the barrier and shown that it is a mucin glycoprotein (Otogelin-like or Otogl) with similar sequence, domain organization, and structural properties to human gel-forming mucins. This mucin forms the structural basis of a surface barrier (∼6 μm thick), which is depleted through knockdown of Otogl. Crucially, Otogl knockdown leads to susceptibility to infection by the opportunistic pathogen Aeromonas hydrophila To more accurately reflect its structure, tissue localization, and function, we have renamed Otogl as Xenopus Skin Mucin, or MucXS. Our findings characterize an accessible and tractable model system to define mucus barrier function and host-microbe interactions.

PubMed ID: 29311327
PMC ID: PMC5789918
Article link: Proc Natl Acad Sci U S A
Grant support: [+]
Genes referenced: mrc1 muc5ac muc5b ncor2 otog otogl2 pts vwf
Antibodies: Otogl Ab1
Morpholinos: otogl MO1


Article Images: [+] show captions
References [+] :
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