Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-54467
Dev Cell January 1, 2017; 43 (6): 744-762.e11.

Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways.

Sigg MA , Menchen T , Lee C , Johnson J , Jungnickel MK , Choksi SP , Garcia G , Busengdal H , Dougherty GW , Pennekamp P , Werner C , Rentzsch F , Florman HM , Krogan N , Wallingford JB , Omran H , Reiter JF .


Abstract
Cilia are organelles specialized for movement and signaling. To infer when during evolution signaling pathways became associated with cilia, we characterized the proteomes of cilia from sea urchins, sea anemones, and choanoflagellates. We identified 437 high-confidence ciliary candidate proteins conserved in mammals and discovered that Hedgehog and G-protein-coupled receptor pathways were linked to cilia before the origin of bilateria and transient receptor potential (TRP) channels before the origin of animals. We demonstrated that candidates not previously implicated in ciliary biology localized to cilia and further investigated ENKUR, a TRP channel-interacting protein identified in the cilia of all three organisms. ENKUR localizes to motile cilia and is required for patterning the left-right axis in vertebrates. Moreover, mutation of ENKUR causes situs inversus in humans. Thus, proteomic profiling of cilia from diverse eukaryotes defines a conserved ciliary proteome, reveals ancient connections to signaling, and uncovers a ciliary protein that underlies development and human disease.

PubMed ID: 29257953
PMC ID: PMC5752135
Article link: Dev Cell
Grant support: [+]

Species referenced: Xenopus
Genes referenced: actb arl13b cep164 enkur pitx2 rpe shh tuba4b
GO keywords: G-protein coupled receptor signaling pathway [+]
Antibodies: Tuba4b Ab5 actb Ab4
Morpholinos: enkur MO1

Disease Ontology terms: situs inversus

Article Images: [+] show captions
References [+] :
, , Pubmed