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XB-ART-54471
Sci Rep January 1, 2018; 8 (1): 1204.

Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling.

Vacca B , Sanchez-Heras E , Steed E , Busson SL , Balda MS , Ohnuma SI , Sasai N , Mayor R , Matter K .


Abstract
Tight junctions are required for the formation of tissue barriers and function as suppressors of signalling mechanisms that control gene expression and cell behaviour; however, little is known about the physiological and developmental importance of such signalling functions. Here, we demonstrate that depletion of MarvelD3, a transmembrane protein of tight junctions, disrupts neural crest formation and, consequently, development of neural crest-derived tissues during Xenopus embryogenesis. Using embryos and explant cultures combined with a small molecule inhibitor or mutant mRNAs, we show that MarvelD3 is required to attenuate JNK signalling during neural crest induction and that inhibition of JNK pathway activation is sufficient to rescue the phenotype induced by MarvelD3 depletion. Direct JNK stimulation disrupts neural crest development, supporting the importance of negative regulation of JNK. Our data identify the junctional protein MarvelD3 as an essential regulator of early vertebrate development and neural crest induction and, thereby, link tight junctions to the control and timing of JNK signalling during early development.

PubMed ID: 29352236
PMC ID: PMC5775312
Article link: Sci Rep
Grant support: [+]
Genes referenced: cdh1 chrd.1 foxd3 gsc jun krt12.4 mapk8 marveld3 odc1 pax3 six1 snai1 snai2 sox2 tbxt twist1 wnt8a
GO keywords: neural crest formation [+]
Antibodies: Cdh1 Ab3 Jun Ab3
Morpholinos: marveld3 MO1 marveld3 MO2


Article Images: [+] show captions
References:

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