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XB-ART-54472
Elife January 1, 2018; 7

FGF mediated MAPK and PI3K/Akt Signals make distinct contributions to pluripotency and the establishment of Neural Crest.

Geary L , LaBonne C .


Abstract
Early vertebrate embryos possess cells with the potential to generate all embryonic cell types. While this pluripotency is progressively lost as cells become lineage restricted, Neural Crest cells retain broad developmental potential. Here, we provide novel insights into signals essential for both pluripotency and neural crest formation in Xenopus. We show that FGF signaling controls a subset of genes expressed by pluripotent blastula cells, and find a striking switch in the signaling cascades activated by FGF signaling as cells lose pluripotency and commence lineage restriction. Pluripotent cells display and require Map Kinase signaling, whereas PI3 Kinase/Akt signals increase as developmental potential is restricted, and are required for transit to certain lineage restricted states. Importantly, retaining a high Map Kinase/low Akt signaling profile is essential for establishing Neural Crest stem cells. These findings shed important light on the signal-mediated control of pluripotency and the molecular mechanisms governing genesis of Neural Crest.

PubMed ID: 29350613
PMC ID: PMC5790379
Article link: Elife
Grant support: [+]
Genes referenced: a2m akt1 chrd.1 fgfr4 foxd3 id3 krt12.4 mapk1 myc myod1 nrp1 pax3 pik3ca pik3cg pou5f3.3 snai1 snai2 sox11 sox17a sox17b.2 sox2 sox3 sox5 sox9 tbxt trim29 ventx2.2 zic1 zic2
GO keywords: ectodermal cell fate determination [+]


Article Images: [+] show captions
References:

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