XB-ART-54475PLoS One January 1, 2018; 13 (1): e0191470.
Cloning and spatiotemporal expression of Xenopus laevis Apolipoprotein CI.
Apolipoprotein CI (ApoCI) belongs to the Apolipoprotein superfamily, members of which are involved in lipid transport, uptake and homeostasis. Excessive ApoCI has been implicated in atherosclerosis and Alzheimer''s disease in humans. In this study we report the isolation of Xenopus laevis apoCI and describe the expression pattern of this gene during early development, using reverse transcription polymerase chain reaction and whole mount in situ hybridization. Xenopus apoCI is enriched in the dorsal ectoderm during gastrulation, and is subsequently expressed in sensory placodes, neural tube and cranial neural crest. These data suggest as yet uncharacterized roles for ApoCI during early vertebrate embryogenesis.
PubMed ID: 29346450
PMC ID: PMC5773212
Article link: PLoS One
Genes referenced: pax6 otx2 snai2 apoc1 chrd.1 clock frzb2
Article Images: [+] show captions
|Fig 1. Sequence analysis of Xenopus apoCI.(A) Nucleotide sequence and deduced amino acid sequence of Xenopus apoCI (GenBank accession number: GU562893). Predicted signal sequence of Xenopus ApoCI spans residues 1–20. (B) Phylogenetic tree depicting evolutionary relationship of human Apolipoproteins AI, AII, AIV, B, CI, CII, CIII and E to Xenopus Apolipoprotein CI. The tree was constructed using the UPGMA distance matrix method from CLC Bio Workbench. Numbers at nodes indicate branch length.|
|Fig 2. Temporal expression of apoCI mRNA.RT-PCR of RNA isolated from whole embryos between stage 4 and stage 32. Chordin serves as a staging control and Ornithine decarboxylase (ODC) as a control for input RNA levels.|
|Fig 3. Spatial expression of apoCI mRNA.Vegetal (A, B) and lateral (A’, B’) views of stage 10 (A, A’) and stage 11 (B, B’) embryos, respectively; apoCI transcript is localized to the dorsal ectoderm. Dotted lines in A, A’ mark the dorsal blastopore lip and dotted lines in B, B’ indicate the blastopore. Dorsal is to left in A, A’, B; dorsal is at 10 o’clock in B’. (C, D) Anterior views of apoCI expression in stage 13 and stage 15 embryos, respectively (dorsal to the top). Dotted line in D marks the pan-placodal primordium. (C’, D’) Dorsal views of embryos in C and D, respectively; anterior is down. (E) Anterior view of a stage 16 embryo; the anterior crescent of apoCI expression has expanded laterally. (F-L) Dorsoanterior views of stage 15 embryos; anterior is down and dorsal is to the top. (F) apoCI expression. (G) slug expression. (H) apoCI (purple) and slug (light blue) coexpression; arrows indicate region of overlap (cranial neural crest). (I) pax6 expression. (J) apoCI (purple) and pax6 (light blue) coexpression. (K) otxA expression. (L) apoCI (purple) and otxA (light blue) coexpression. (M, N) Anterior (M, dorsal is to the top) and dorsal (N, anterior is down) views showing apoCI expression in a stage 21 embryo. (O) Transverse section revealing expression of apoCI in the dorsal neural tube at stage 22; star indicates the neural tube. (P, Q) Lateral views of apoCI expression in stage 27 and stage 33 embryos, respectively; anterior is to the left. Arrow in Q marks liver primordium. No signal was detected with an apoCI sense strand probe (data not shown).|
|apoCI (apolipoprotein C-I) gene expression in X. laeis embryo, NF stage 21, as assayed via in situ hybridization, anterior view, dorsal up.|
|apoCI (apolipoprotein C-I) gene expression in X. laevis embryo, NF stage 22, as assayed via in situ hybridization, anterior view, dorsal up.|
|apoCI (apolipoprotein C-I) expression in X. laevis embryo, transverse section through trunk region, NF stage 22, dorsal up, revealing expression of apoCI in the dorsal neural tube (aka roof plate; star indicates the neural tube).|
|apoCI (apolipoprotein C-I) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, lateral view, anterior left, dorsal up.|
|apoCI (apolipoprotein C-I) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 33 & 34, lateral view, anterior left, dorsal up.|