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Zitouni S
,
Francia ME
,
Leal F
,
Montenegro Gouveia S
,
Nabais C
,
Duarte P
,
Gilberto S
,
Brito D
,
Moyer T
,
Kandels-Lewis S
,
Ohta M
,
Kitagawa D
,
Holland AJ
,
Karsenti E
,
Lorca T
,
Lince-Faria M
,
Bettencourt-Dias M
.
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Centrioles are essential for the assembly of both centrosomes and cilia. Centriole biogenesis occurs once and only once per cell cycle and is temporally coordinated with cell-cycle progression, ensuring the formation of the right number of centrioles at the right time. The formation of new daughter centrioles is guided by a pre-existing, mother centriole. The proximity between mother and daughter centrioles was proposed to restrict new centriole formation until they separate beyond a critical distance. Paradoxically, mother and daughter centrioles overcome this distance in early mitosis, at a time when triggers for centriole biogenesis Polo-like kinase 4 (PLK4) and its substrate STIL are abundant. Here we show that in mitosis, the mitotic kinase CDK1-CyclinB binds STIL and prevents formation of the PLK4-STIL complex and STIL phosphorylation by PLK4, thus inhibiting untimely onset of centriole biogenesis. After CDK1-CyclinB inactivation upon mitotic exit, PLK4 can bind and phosphorylate STIL in G1, allowing pro-centriole assembly in the subsequent S phase. Our work shows that complementary mechanisms, such as mother-daughter centriole proximity and CDK1-CyclinB interaction with centriolar components, ensure that centriole biogenesis occurs once and only once per cell cycle, raising parallels to the cell-cycle regulation of DNA replication and centromere formation.
Arquint,
STIL microcephaly mutations interfere with APC/C-mediated degradation and cause centriole amplification.
2014, Pubmed
Arquint,
STIL microcephaly mutations interfere with APC/C-mediated degradation and cause centriole amplification.
2014,
Pubmed
Arquint,
Cell-cycle-regulated expression of STIL controls centriole number in human cells.
2012,
Pubmed
Arquint,
STIL binding to Polo-box 3 of PLK4 regulates centriole duplication.
2015,
Pubmed
Bettencourt-Dias,
SAK/PLK4 is required for centriole duplication and flagella development.
2005,
Pubmed
Bettencourt-Dias,
Centrosome biogenesis and function: centrosomics brings new understanding.
2007,
Pubmed
Bishop,
Design of allele-specific inhibitors to probe protein kinase signaling.
1998,
Pubmed
Bishop,
A chemical switch for inhibitor-sensitive alleles of any protein kinase.
2000,
Pubmed
Chen,
CDK prevents Mcm2-7 helicase loading by inhibiting Cdt1 interaction with Orc6.
2011,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Dzhindzhev,
Plk4 phosphorylates Ana2 to trigger Sas6 recruitment and procentriole formation.
2014,
Pubmed
Dzhindzhev,
Asterless is a scaffold for the onset of centriole assembly.
2010,
Pubmed
Eckerdt,
Identification of a polo-like kinase 4-dependent pathway for de novo centriole formation.
2011,
Pubmed
,
Xenbase
Enserink,
An overview of Cdk1-controlled targets and processes.
2010,
Pubmed
Fu,
The centrosome and its duplication cycle.
2015,
Pubmed
,
Xenbase
Gönczy,
Towards a molecular architecture of centriole assembly.
2012,
Pubmed
Habedanck,
The Polo kinase Plk4 functions in centriole duplication.
2005,
Pubmed
Hengeveld,
Development of a chemical genetic approach for human aurora B kinase identifies novel substrates of the chromosomal passenger complex.
2012,
Pubmed
Hinchcliffe,
Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts.
1999,
Pubmed
,
Xenbase
Holland,
Polo-like kinase 4 kinase activity limits centrosome overduplication by autoregulating its own stability.
2010,
Pubmed
Jakobsen,
Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
2011,
Pubmed
Khodjakov,
De novo formation of centrosomes in vertebrate cells arrested during S phase.
2002,
Pubmed
Kim,
Hierarchical recruitment of Plk4 and regulation of centriole biogenesis by two centrosomal scaffolds, Cep192 and Cep152.
2013,
Pubmed
Kodani,
Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
2015,
Pubmed
Koff,
Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle.
1992,
Pubmed
Kratz,
Plk4-dependent phosphorylation of STIL is required for centriole duplication.
2015,
Pubmed
Leidel,
SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells.
2005,
Pubmed
Lorca,
Constant regulation of both the MPF amplification loop and the Greatwall-PP2A pathway is required for metaphase II arrest and correct entry into the first embryonic cell cycle.
2010,
Pubmed
,
Xenbase
Matsumoto,
Cyclin-dependent kinase 2 (Cdk2) is required for centrosome duplication in mammalian cells.
1999,
Pubmed
Mazia,
The Multiplicity of the Mitotic Centers and the Time-Course of Their Duplication and Separation.
1960,
Pubmed
McIntosh,
Dormant origins, the licensing checkpoint, and the response to replicative stresses.
2012,
Pubmed
Meraldi,
Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A.
1999,
Pubmed
Moyer,
Binding of STIL to Plk4 activates kinase activity to promote centriole assembly.
2015,
Pubmed
Nguyen,
Cyclin-dependent kinases prevent DNA re-replication through multiple mechanisms.
2001,
Pubmed
Ohta,
Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole.
2014,
Pubmed
Rodrigues-Martins,
Revisiting the role of the mother centriole in centriole biogenesis.
2007,
Pubmed
Shukla,
Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation.
2015,
Pubmed
Sillibourne,
Autophosphorylation of polo-like kinase 4 and its role in centriole duplication.
2010,
Pubmed
Silva,
Cdk activity couples epigenetic centromere inheritance to cell cycle progression.
2012,
Pubmed
Sluder,
Centriole number and the reproductive capacity of spindle poles.
1985,
Pubmed
Steere,
Centrosome amplification in CHO and DT40 cells by inactivation of cyclin-dependent kinases.
2011,
Pubmed
Tokmakov,
Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs.
2010,
Pubmed
,
Xenbase
Tsou,
Mechanism limiting centrosome duplication to once per cell cycle.
2006,
Pubmed
,
Xenbase
Vassilev,
Cell cycle synchronization at the G2/M phase border by reversible inhibition of CDK1.
2006,
Pubmed
Vidwans,
Anomalous centriole configurations are detected in Drosophila wing disc cells upon Cdk1 inactivation.
2003,
Pubmed
Wong,
Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.
2015,
Pubmed
Zitouni,
Polo-like kinases: structural variations lead to multiple functions.
2014,
Pubmed
