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Chem Senses
2017 May 01;424:333-341. doi: 10.1093/chemse/bjx011.
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Functional and Nonfunctional Forms of CquiOR91, an Odorant Selectivity Subunit of Culex quinquefasciatus.
Hughes DT
,
Pelletier J
,
Rahman S
,
Chen S
,
Leal WS
,
Luetje CW
.
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In Culex quinquefasciatus, CquiOR91 is the ortholog of 2 larvae-specific odorant receptors (ORs) from Anopheles gambiae (Agam\Or40, previously shown to respond to several odorant ligands including the broad-spectrum repellent N,N-diethyl-3-methylbenzamide, DEET) and Aedes aegypti (Aaeg\Or40). When we cloned full-length CquiOR91 from a Culex quinquefasciatus larval head RNA sample, we found 2 alleles of this OR, differing at 9 residues. Functional analysis using the Xenopus oocyte expression system and 2-electrode voltage clamp electrophysiology revealed one allele (CquiOR91.1) to be nonfunctional, whereas the other allele (CquiOR91.2) was functional. Receptors formed by CquiOR91.2 and Cqui\Orco responded to (-)-fenchone, (+)-fenchone, and DEET, similar to what has been reported for Agam\Or40. We also identified 5 novel odorant ligands for the CquiOR91.2 + Cqui\Orco receptor: 2-isobutylthiazole, veratrole, eucalyptol, d-camphor, and safranal, with safranal being the most potent. To explore possible reasons for the lack of function for CquiOR91.1, we generated a series of mutant CquiOR91.2 subunits, in which the residue at each of the 9 polymorphic residue positions was changed from what occurs in CquiOR91.2 to what occurs in CquiOR91.1. Eight of the 9 mutant versions of CquiOR91.2 formed functional receptors, responding to (-)-fenchone. Only the CquiOR91.2 Y183C mutant was nonfunctional. The reverse mutation (C183Y) conferred function on CquiOR91.1 , which became responsive to (-)-fenchone and safranal. These results indicate that the "defect" in CquiOR91.1 that prevents function is the cysteine at position 183.
Benton,
Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo.
2006, Pubmed
Benton,
Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo.
2006,
Pubmed
Benton,
Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila.
2009,
Pubmed
Bohbot,
Molecular characterization of the Aedes aegypti odorant receptor gene family.
2007,
Pubmed
Carey,
Odorant reception in the malaria mosquito Anopheles gambiae.
2010,
Pubmed
Chen,
Identification of new agonists and antagonists of the insect odorant receptor co-receptor subunit.
2012,
Pubmed
,
Xenbase
Chen,
Phenylthiophenecarboxamide antagonists of the olfactory receptor co-receptor subunit from a mosquito.
2013,
Pubmed
,
Xenbase
Chen,
Trace amines inhibit insect odorant receptor function through antagonism of the co-receptor subunit.
2014,
Pubmed
Combet,
NPS@: network protein sequence analysis.
2000,
Pubmed
DeGennaro,
orco mutant mosquitoes lose strong preference for humans and are not repelled by volatile DEET.
2013,
Pubmed
Ditzen,
Insect odorant receptors are molecular targets of the insect repellent DEET.
2008,
Pubmed
,
Xenbase
Erdelyan,
Functional validation of the carbon dioxide receptor genes in Aedes aegypti mosquitoes using RNA interference.
2012,
Pubmed
Franca,
First International Workshop on Zika Virus Held by Oswaldo Cruz Foundation FIOCRUZ in Northeast Brazil March 2016 - A Meeting Report.
2016,
Pubmed
Gibson,
Visual and olfactory responses of haematophagous Diptera to host stimuli.
1999,
Pubmed
Hallem,
The spatial code for odors is changed by conditioning.
2004,
Pubmed
Hallem,
Olfaction: mosquito receptor for human-sweat odorant.
2004,
Pubmed
Hallem,
The molecular basis of odor coding in the Drosophila antenna.
2004,
Pubmed
Hallem,
Coding of odors by a receptor repertoire.
2006,
Pubmed
Hughes,
Odorant receptor from the southern house mosquito narrowly tuned to the oviposition attractant skatole.
2010,
Pubmed
,
Xenbase
Jones,
Functional conservation of an insect odorant receptor gene across 250 million years of evolution.
2005,
Pubmed
Krieger,
A candidate olfactory receptor subtype highly conserved across different insect orders.
2003,
Pubmed
Larsson,
Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction.
2004,
Pubmed
Leal,
Differential expression of olfactory genes in the southern house mosquito and insights into unique odorant receptor gene isoforms.
2013,
Pubmed
Lee,
High degree of single nucleotide polymorphisms in California Culex pipiens (Diptera: Culicidae) sensu lato.
2012,
Pubmed
Li,
A broadly tuned mouse odorant receptor that detects nitrotoluenes.
2012,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Lu,
Odor coding in the maxillary palp of the malaria vector mosquito Anopheles gambiae.
2007,
Pubmed
Luetje,
Functional assay of mammalian and insect olfactory receptors using Xenopus oocytes.
2013,
Pubmed
,
Xenbase
McAbee,
Pyrethroid tolerance in Culex pipiens pipiens var molestus from Marin County, California.
2004,
Pubmed
McMeniman,
Multimodal integration of carbon dioxide and other sensory cues drives mosquito attraction to humans.
2014,
Pubmed
Nakagawa,
Insect sex-pheromone signals mediated by specific combinations of olfactory receptors.
2005,
Pubmed
,
Xenbase
Neuhaus,
Odorant receptor heterodimerization in the olfactory system of Drosophila melanogaster.
2005,
Pubmed
Nichols,
Subunit contributions to insect olfactory receptor function: channel block and odorant recognition.
2011,
Pubmed
,
Xenbase
Nichols,
Transmembrane segment 3 of Drosophila melanogaster odorant receptor subunit 85b contributes to ligand-receptor interactions.
2010,
Pubmed
,
Xenbase
Nugent,
Transmembrane protein topology prediction using support vector machines.
2009,
Pubmed
Pask,
Heteromeric Anopheline odorant receptors exhibit distinct channel properties.
2011,
Pubmed
Pelletier,
An odorant receptor from the southern house mosquito Culex pipiens quinquefasciatus sensitive to oviposition attractants.
2010,
Pubmed
,
Xenbase
Pitts,
A highly conserved candidate chemoreceptor expressed in both olfactory and gustatory tissues in the malaria vector Anopheles gambiae.
2004,
Pubmed
Sato,
Insect olfactory receptors are heteromeric ligand-gated ion channels.
2008,
Pubmed
,
Xenbase
Silbering,
Ir40a neurons are not DEET detectors.
2016,
Pubmed
Sánchez-Gracia,
Molecular evolution of the major chemosensory gene families in insects.
2009,
Pubmed
Vosshall,
A unified nomenclature system for the insect olfactory coreceptor.
2011,
Pubmed
Vosshall,
Molecular architecture of smell and taste in Drosophila.
2007,
Pubmed
Wang,
Molecular basis of odor coding in the malaria vector mosquito Anopheles gambiae.
2010,
Pubmed
,
Xenbase
Wanner,
A honey bee odorant receptor for the queen substance 9-oxo-2-decenoic acid.
2007,
Pubmed
,
Xenbase
Wicher,
Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels.
2008,
Pubmed
Xia,
The molecular and cellular basis of olfactory-driven behavior in Anopheles gambiae larvae.
2008,
Pubmed
,
Xenbase
Xu,
Silent, generic and plant kairomone sensitive odorant receptors from the Southern house mosquito.
2013,
Pubmed
,
Xenbase
Xu,
Mosquito odorant receptor for DEET and methyl jasmonate.
2014,
Pubmed
,
Xenbase
Xu,
Probing insect odorant receptors with their cognate ligands: insights into structural features.
2013,
Pubmed
,
Xenbase