Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-54812
Dev Growth Differ April 1, 2018; 60 (3): 158-173.

Coordinated regulation of the dorsal-ventral and anterior-posterior patterning of Xenopus embryos by the BTB/POZ zinc finger protein Zbtb14.

Takebayashi-Suzuki K , Konishi H , Miyamoto T , Nagata T , Uchida M , Suzuki A .


Abstract
During early vertebrate embryogenesis, bone morphogenetic proteins (BMPs) belonging to the transforming growth factor-β (TGF-β) family of growth factors play a central role in dorsal-ventral (DV) patterning of embryos, while other growth factors such as Wnt and fibroblast growth factor (FGF) family members regulate formation of the anterior-posterior (AP) axis. Although the establishment of body plan is thought to require coordinated formation of the DV and AP axes, the mechanistic details underlying this coordination are not well understood. Here, we show that a Xenopus homologue of zbtb14 plays an essential role in the regulation of both DV and AP patterning during early Xenopus development. We show that overexpression of Zbtb14 promotes neural induction and inhibits epidermal differentiation, thereby regulating DV patterning. In addition, Zbtb14 promotes the formation of posterior neural tissue and suppresses anterior neural development. Consistent with this, knock-down experiments show that Zbtb14 is required for neural development, especially for the formation of posterior neural tissues. Mechanistically, Zbtb14 reduces the levels of phosphorylated Smad1/5/8 to suppress BMP signaling and induces an accumulation of β-Catenin to promote Wnt signaling. Collectively, these results suggest that Zbtb14 plays a crucial role in the formation of DV and AP axes by regulating both the BMP and Wnt signaling pathways during early Xenopus embryogenesis.

PubMed ID: 29664133
Article link: Dev Growth Differ

Genes referenced: atp8b1 bmpr1b cdx2 egr2 emb en2 hoxb9 krt12.4 myc myod1 ncam1 otx2 pax6 rax smad1 smad3 smad6.2 smad7 smurf1 smurf2 sox2 tubb2b zbtb14
GO keywords: Wnt signaling pathway [+]


Article Images: [+] show captions


Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.12.0


Major funding for Xenbase is provided by grant P41 HD064556