XB-ART-54823
Science
2018 Feb 09;3596376:. doi: 10.1126/science.aao6135.
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A pathway for mitotic chromosome formation.
Gibcus JH
,
Samejima K
,
Goloborodko A
,
Samejima I
,
Naumova N
,
Nuebler J
,
Kanemaki MT
,
Xie L
,
Paulson JR
,
Earnshaw WC
,
Mirny LA
,
Dekker J
.
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Mitotic chromosomes fold as compact arrays of chromatin loops. To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed. During prometaphase, ~80-kb inner loops are nested within ~400-kb outer loops. The loop array acquires a helical arrangement with consecutive loops emanating from a central "spiral staircase" condensin scaffold. The size of helical turns progressively increases to ~12 megabases during prometaphase. Acute depletion of condensin I or II shows that nested loops form by differential action of the two condensins, whereas condensin II is required for helical winding.
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???displayArticle.grants??? [+]
R01 GM114190 NIGMS NIH HHS , R01 HG003143 NHGRI NIH HHS , Howard Hughes Medical Institute , U54 DK107980 NIDDK NIH HHS , Wellcome Trust , U54 CA193419 NCI NIH HHS , 107022 Wellcome Trust
Species referenced: Xenopus laevis
Genes referenced: cdk1
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