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XB-ART-55080
Dev Cell January 1, 2018; 45 (5): 565-579.e3.

Redistribution of Adhesive Forces through Src/FAK Drives Contact Inhibition of Locomotion in Neural Crest.

Roycroft A , Szabó A , Bahm I , Daly L , Charras G , Parsons M , Mayor R .


Abstract
Contact inhibition of locomotion is defined as the behavior of cells to cease migrating in their former direction after colliding with another cell. It has been implicated in multiple developmental processes and its absence has been linked to cancer invasion. Cellular forces are thought to govern this process; however, the exact role of traction through cell-matrix adhesions and tension through cell-cell adhesions during contact inhibition of locomotion remains unknown. Here we use neural crest cells to address this and show that cell-matrix adhesions are rapidly disassembled at the contact between two cells upon collision. This disassembly is dependent upon the formation of N-cadherin-based cell-cell adhesions and driven by Src and FAK activity. We demonstrate that the loss of cell-matrix adhesions near the contact leads to a buildup of tension across the cell-cell contact, a step that is essential to drive cell-cell separation after collision.

PubMed ID: 29870718
PMC ID: PMC5988567
Article link: Dev Cell
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cdh1 cdh2 ctnnd1 fn1 ptk2 pxn sox10 src twist1 vcl
GO keywords: neural crest cell migration [+]
Antibodies: Cdh1 Ab1 Cdh2 Ab3 Cdh2 Ab4 Pxn Ab2 Src Ab3 Vcl Ab1
Morpholinos: cdh2 MO1


Article Images: [+] show captions
References [+] :
ABERCROMBIE, Interference microscope studies of cell contacts in tissue culture. 2000, Pubmed