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XB-ART-55100
Development January 1, 2018; 145 (15):

Histone deacetylase activity has an essential role in establishing and maintaining the vertebrate neural crest.

Rao A , LaBonne C .


Abstract
The neural crest, a progenitor population that drove vertebrate evolution, retains the broad developmental potential of the blastula cells it is derived from, even as neighboring cells undergo lineage restriction. The mechanisms that enable these cells to preserve their developmental potential remain poorly understood. Here, we explore the role of histone deacetylase (HDAC) activity in this process in Xenopus We show that HDAC activity is essential for the formation of neural crest, as well as for proper patterning of the early ectoderm. The requirement for HDAC activity initiates in naïve blastula cells; HDAC inhibition causes loss of pluripotency gene expression and blocks the ability of blastula stem cells to contribute to lineages of the three embryonic germ layers. We find that pluripotent naïve blastula cells and neural crest cells are both characterized by low levels of histone acetylation, and show that increasing HDAC1 levels enhance the ability of blastula cells to be reprogrammed to a neural crest state. Together, these findings elucidate a previously uncharacterized role for HDAC activity in establishing the neural crest stem cell state.

PubMed ID: 30002130
PMC ID: PMC6110147
Article link: Development
Grant support: [+]
Genes referenced: a2m chrd.1 ets1 foxd3 hdac1 hdac2 hdac3 id3 krt12.4 lin28a msx2 myc myod1 nrp1 olig2 pax3 pou5f3.1 pou5f3.2 pou5f3.3 prdm1 six1 snai2 sox11 sox17a sox17b.2 sox2 sox3 tbxt tfap2a tubb4b vegt ventx2.2 zic1
GO keywords: neural crest cell development [+]
Antibodies: Acetylated H3f3a Ab20 Somite Ab1 h3-3a Ab39
Morpholinos: hdac1 MO2


Article Images: [+] show captions
References [+] :
Almouzni, Histone acetylation influences both gene expression and development of Xenopus laevis. 1994, Pubmed, Xenbase


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