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Mech Dev January 1, 2018; 154 170-178.

Ric-8A, a GEF for heterotrimeric G-proteins, controls cranial neural crest cell polarity during migration.

Leal JI , Villaseca S , Beyer A , Toro-Tapia G , Torrejón M .

The neural crest (NC) is a transient embryonic cell population that migrates extensively during development. Ric-8A, a guanine nucleotide exchange factor (GEF) for different Gα subunits regulates cranial NC (CNC) cell migration in Xenopus through a mechanism that still remains to be elucidated. To properly migrate, CNC cells establish an axis of polarization and undergo morphological changes to generate protrusions at the leading edge and retraction of the cell rear. Here, we aim to study the role of Ric-8A in cell polarity during CNC cell migration by examining whether its signaling affects the localization of GTPase activity in Xenopus CNC using GTPase-based probes in live cells and aPKC and Par3 as polarity markers. We show that the levels of Ric-8A are critical during migration and affect the localization of polarity markers and the subcellular localization of GTPase activity, suggesting that Ric-8A, probably through heterotrimeric G-protein signaling, regulates cell polarity during CNC migration.

PubMed ID: 30016646
Article link: Mech Dev

Genes referenced: h2bc21 pard3 prkci rac1 rhoa ric8a snai2 sox2 twist1
GO keywords: neural crest cell migration [+]
Antibodies: ric8a Ab1
Morpholinos: ric8a MO1

Article Images: [+] show captions

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