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XB-ART-55167
Mech Dev January 1, 2018; 154 219-239.

Gli2 is required for the induction and migration of Xenopus laevis neural crest.

Cerrizuela S , Vega-López GA , Palacio MB , Tríbulo C , Aybar MJ .


Abstract
The neural crest (NC) is a multipotent migratory embryonic population that is formed during late gastrulation and gives rise to a wide array of derivatives, including cells from the peripheral nervous system (PNS), the craniofacial bones and cartilages, peripheral glial cells, and melanocyte cells, among others. In this work we analyzed the role of the Hedgehog signaling pathway effector gli2 in Xenopus NC. We provide evidence that the gli2 gene is expressed in the prospective, premigratory and migratory NC. The use of a specific morpholino against gli2 and the pharmacological specific inhibitor GANT61 in different experimental approaches allowed us to determine that gli2 is required for the induction and specification of NC cells as a transcriptional activator. Moreover, gli2 also acts by reducing apoptosis in the NC without affecting its cell proliferation status. We also demonstrated that gli2 is required cell-autonomously for NC migration, and for the formation of NC derivatives such as the craniofacial cartilages, melanocytes and the cranial ganglia. Altogether, our results showed that gli2 is a key transcriptional activator to accomplish the proper specification and development of Xenopus NC cells.

PubMed ID: 30086335
Article link: Mech Dev

Genes referenced: gli2 sox2 foxd3 ptch1 snai1 pax3 msx1 snai2 sox9 krt12.4 sox10 tbx2 cdh1 cdh2 dlx5 ednra gli3 hes4 ihh kif11 six1 tuba1cl twist1
GO Terms: neural crest cell migration [+]
Antibodies: Tuba1a Ab1 ptch1 Ab2
Morpholinos: ednra MO1 gli2 MO2 gli3 MO1 ihh MO1 kif11 MO1


Article Images: [+] show captions


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