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XB-ART-55177
Dev Biol January 1, 2018; 442 (2): 276-287.

Katanin-like protein Katnal2 is required for ciliogenesis and brain development in Xenopus embryos.

Willsey HR , Walentek P , Exner CRT , Xu Y , Lane AB , Harland RM , Heald R , Santama N .


Abstract
Microtubule remodeling is critical for cellular and developmental processes underlying morphogenetic changes and for the formation of many subcellular structures. Katanins are conserved microtubule severing enzymes that are essential for spindle assembly, ciliogenesis, cell division, and cellular motility. We have recently shown that a related protein, Katanin-like 2 (KATNAL2), is similarly required for cytokinesis, cell cycle progression, and ciliogenesis in cultured mouse cells. However, its developmental expression pattern, localization, and in vivo role during organogenesis have yet to be characterized. Here, we used Xenopus embryos to reveal that Katnal2 (1) is expressed broadly in ciliated and neurogenic tissues throughout embryonic development; (2) is localized to basal bodies, ciliary axonemes, centrioles, and mitotic spindles; and (3) is required for ciliogenesis and brain development. Since human KATNAL2 is a risk gene for autism spectrum disorders, our functional data suggest that Xenopus may be a relevant system for understanding the relationship of mutations in this gene to autism and the underlying molecular mechanisms of pathogenesis.

PubMed ID: 30096282
PMC ID: PMC6143417
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actb arhgef5 cfp drosha hhex katnal2 pcna slc17a7 slc45a2 smn1 snai2 tub tuba4b tubb
GO keywords: cilium [+]
Antibodies: Glutamylated Tubulin Ab1 Tuba4a Ab3 Tuba4b Ab5 Tubb Ab1 katnal2 Ab1 katnal2 Ab2 slc17a7 Ab2
Morpholinos: katnal2 MO1

Disease Ontology terms: autism spectrum disorder

Article Images: [+] show captions
References [+] :
Antoniades, Making the connection: ciliary adhesion complexes anchor basal bodies to the actin cytoskeleton. 2014, Pubmed