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Methods Mol Biol January 1, 2018; 1865 251-263.

Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis.

The Xenopus embryonic epidermis serves as a model to investigate the development, cell biology, and regeneration of vertebrate mucociliary epithelia. Its fast development as well as the ease of manipulation and analysis in this system facilitate novel approaches and sophisticated experiments addressing the principle mechanisms of mucociliary signaling, transcriptional regulation, and morphogenesis. This protocol describes how cell type composition can be manipulated and analyzed, and how mucociliary organoids can be generated and used for "omics"-type of experiments.

PubMed ID: 30151772
Article link: Methods Mol Biol

Genes referenced: atp6v1e1 foxa1 foxi1 mcidas tph1 tuba4b
Morpholinos: foxa1 MO2 mcidas MO1 mcidas MO2

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