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Front Physiol
2018 Jan 01;9:1188. doi: 10.3389/fphys.2018.01188.
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Proceeding From in vivo Functions of Pheromone Receptors: Peripheral-Coding Perception of Pheromones From Three Closely Related Species, Helicoverpa armigera, H. assulta, and Heliothis virescens.
Wang B
,
Liu Y
,
Wang GR
.
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Three closely related species, Helicoverpa armigera, H. assulta, and Heliothis virescens from Lepidoptera Noctuidae, are used as a model system for exploring sexual communication and species isolation. Pheromone receptors (PRs) previously discovered in model moth species include seven in H. armigera, six in H. assulta, and six in H. virescens. PRs named OR6, OR13, and OR16 among these species were found to be functional, characterized by an in vitro Xenopus oocytes system. Using an in vivo transgenic fly system, functional assays of OR6, OR13, and OR16 clades from three closely related Noctuidae species showed that OR13 function was highly conserved, whereas OR6 and OR16 exhibited functional divergence. Similar results were produced from assays in the Xenopus oocytes system. Combined with earlier behavioral results and electrophysiological recordings, we found corresponding relationships among pheromones, PRs, and neurons at the periphery sensory system of each species. Our results provide vital information at the neuronal and molecular level, shedding insight into the sexual communication of closely related species in Lepidoptera.
FIGURE 1. The phylogeny of pheromone receptors (PRs) from seven moth species. (A) Phylogeny and number of PRs identified by species, including Helicoverpa armigera, H. assulta, Heliothis virescens, Bombyx mori, Spodoptera exigua, S. litura and S. littoralis.
(B) A phylogenetic tree of PR genes in different Lepidoptera species. Six clades (I to VI) are shown in this tree representing Orco, OR13, OR11, OR14/15, OR16, and OR6 clades, respectively.
FIGURE 2. Motif analysis of pheromone receptors (PRs) identified from three closely related Lepidoptera species, and the alignment of amino acid sequence of three set of PRs. (A) The eight motif-pattern discovered in nine PRs from Helicoverpa armigera, H. assulta, and Heliothis virescens. (B) The locations of each motif-pattern on the predicted protein sequence from N-terminal to C-terminal. Smaller numbers indicate higher conservation. (C) The alignment of amino acid sequence of clade OR6 from H. armigera, H. assulta, and H. virescens. TM1-TM7 indicates seven transmembrane domains. Harm: H. armigera; Hass: H. assulta; Hvir: H. virescens. (D) The alignment of amino acid sequence of clade OR13 from H. armigera, H. assulta, and H. virescens. (E) The alignment of amino acid sequence of clade OR16 from H. armigera, H. assulta, and H. virescens.
FIGURE 3. Odor coding of pheromone receptors (PRs) from three closely related species. (A) Responses of OR6-expressing neurons in at1 sensilla of transgenic flies. (B) Responses of OR13-expressing neurons in at1 sensilla of transgenic flies. (C) Responses of OR16-expressing neurons in at1 sensilla of transgenic flies. (D) Heatmap of response spectra of PR-expressing neurons in at1 sensilla of transgenic flies.
FIGURE 4. Odor coding of pheromone receptors from three closely related species across concentrations. (A) Dose-responses of OR6-expressing neurons in at1 sensilla of transgenic flies. (B) Dose-responses of OR13-expressing neurons in at1 sensilla of transgenic flies. (C) Dose-responses of OR16-expressing neurons in at1 sensilla of transgenic flies.
Almaas,
Heliothis virescens: Response characteristics of receptor neurons in Sensilla Trichodea type 1 and type 2.
1991, Pubmed
Almaas,
Heliothis virescens: Response characteristics of receptor neurons in Sensilla Trichodea type 1 and type 2.
1991,
Pubmed
Baker,
Nearest neural neighbors: moth sex pheromone receptors HR11 and HR13.
2009,
Pubmed
Baker,
A comparison of responses from olfactory receptor neurons of Heliothis subflexa and Heliothis virescens to components of their sex pheromone.
2004,
Pubmed
Benton,
An essential role for a CD36-related receptor in pheromone detection in Drosophila.
2007,
Pubmed
Benton,
Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo.
2006,
Pubmed
Carey,
Odorant reception in the malaria mosquito Anopheles gambiae.
2010,
Pubmed
Chang,
Sensillar expression and responses of olfactory receptors reveal different peripheral coding in two Helicoverpa species using the same pheromone components.
2016,
Pubmed
Chang,
A Pheromone Antagonist Regulates Optimal Mating Time in the Moth Helicoverpa armigera.
2017,
Pubmed
Cork,
Female sex pheromone of oriental tobacco budworm,Helicoverpa assulta (Guenee) (Lepidoptera: Noctuidae): Identification and field testing.
1992,
Pubmed
Cui,
A receptor-neuron correlate for the detection of attractive plant volatiles in Helicoverpa assulta (Lepidoptera: Noctuidae).
2018,
Pubmed
,
Xenbase
Deng,
HemI: a toolkit for illustrating heatmaps.
2014,
Pubmed
Dobritsa,
Integrating the molecular and cellular basis of odor coding in the Drosophila antenna.
2003,
Pubmed
Gould,
Sexual isolation of male moths explained by a single pheromone response QTL containing four receptor genes.
2010,
Pubmed
Groot,
QTL analysis of sex pheromone blend differences between two closely related moths: Insights into divergence in biosynthetic pathways.
2009,
Pubmed
Groot,
One quantitative trait locus for intra- and interspecific variation in a sex pheromone.
2013,
Pubmed
Groot,
Experimental evidence for interspecific directional selection on moth pheromone communication.
2006,
Pubmed
Grosse-Wilde,
Candidate pheromone receptors provide the basis for the response of distinct antennal neurons to pheromonal compounds.
2007,
Pubmed
Grosse-Wilde,
A pheromone-binding protein mediates the bombykol-induced activation of a pheromone receptor in vitro.
2006,
Pubmed
Hallem,
The molecular basis of odor coding in the Drosophila antenna.
2004,
Pubmed
Heckel,
Smells like a new species: gene duplication at the periphery.
2010,
Pubmed
Jiang,
Sequence similarity and functional comparisons of pheromone receptor orthologs in two closely related Helicoverpa species.
2014,
Pubmed
,
Xenbase
Krieger,
HR11 and HR13 receptor-expressing neurons are housed together in pheromone-responsive sensilla trichodea of male Heliothis virescens.
2009,
Pubmed
Krieger,
Genes encoding candidate pheromone receptors in a moth (Heliothis virescens).
2004,
Pubmed
Kurtovic,
A single class of olfactory neurons mediates behavioural responses to a Drosophila sex pheromone.
2007,
Pubmed
Leary,
Single mutation to a sex pheromone receptor provides adaptive specificity between closely related moth species.
2012,
Pubmed
,
Xenbase
Liu,
Candidate olfaction genes identified within the Helicoverpa armigera Antennal Transcriptome.
2012,
Pubmed
Liu,
Identification and functional characterization of sex pheromone receptors in beet armyworm Spodoptera exigua (Hübner).
2013,
Pubmed
,
Xenbase
Liu,
Functional specificity of sex pheromone receptors in the cotton bollworm Helicoverpa armigera.
2013,
Pubmed
Montagné,
Functional characterization of a sex pheromone receptor in the pest moth Spodoptera littoralis by heterologous expression in Drosophila.
2012,
Pubmed
Nakagawa,
Amino acid residues contributing to function of the heteromeric insect olfactory receptor complex.
2012,
Pubmed
,
Xenbase
Nakagawa,
Insect sex-pheromone signals mediated by specific combinations of olfactory receptors.
2005,
Pubmed
,
Xenbase
Ni,
A genome-scale shRNA resource for transgenic RNAi in Drosophila.
2011,
Pubmed
Sakurai,
Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori.
2004,
Pubmed
,
Xenbase
Stamatakis,
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.
2014,
Pubmed
Syed,
Pheromone reception in fruit flies expressing a moth's odorant receptor.
2006,
Pubmed
Syed,
Bombykol receptors in the silkworm moth and the fruit fly.
2010,
Pubmed
Teal,
Chemical and behavioral analyses of volatile sex pheromone components released by callingHeliothis virescens (F.) females (Lepidoptera: Noctuidae).
1986,
Pubmed
Vetter,
Behavioral responses of maleHeliothis virescens in a sustained-flight tunnel to combinations of seven compounds identified from female sex pheromone glands.
1983,
Pubmed
Vásquez,
Specificity of the receptor for the major sex pheromone component in Heliothis virescens.
2013,
Pubmed
Vásquez,
Differential expression of odorant receptor genes involved in the sexual isolation of two Heliothis moths.
2011,
Pubmed
Wang,
Comparison of research methods for functional characterization of insect olfactory receptors.
2016,
Pubmed
Wang,
Comparative study of sex pheromone composition and biosynthesis in Helicoverpa armigera, H. assulta and their hybrid.
2005,
Pubmed
Wang,
Functional characterization of pheromone receptors in the tobacco budworm Heliothis virescens.
2011,
Pubmed
Wanner,
Female-biased expression of odourant receptor genes in the adult antennae of the silkworm, Bombyx mori.
2007,
Pubmed
Wu,
Specific olfactory neurons and glomeruli are associated to differences in behavioral responses to pheromone components between two Helicoverpa species.
2015,
Pubmed
Xu,
Olfactory perception and behavioral effects of sex pheromone gland components in Helicoverpa armigera and Helicoverpa assulta.
2016,
Pubmed
Yang,
Two single-point mutations shift the ligand selectivity of a pheromone receptor between two closely related moth species.
2017,
Pubmed
,
Xenbase
Zhang,
An overlooked component: (Z)-9-tetradecenal as a sex pheromone in Helicoverpa armigera.
2012,
Pubmed
Zhang,
Antennal transcriptome analysis and comparison of chemosensory gene families in two closely related noctuidae moths, Helicoverpa armigera and H. assulta.
2015,
Pubmed
Zhang,
Functional evolution of a multigene family: orthologous and paralogous pheromone receptor genes in the turnip moth, Agrotis segetum.
2013,
Pubmed
,
Xenbase
Zhang,
Identification and functional characterization of sex pheromone receptors in the common cutworm (Spodoptera litura).
2015,
Pubmed
,
Xenbase
Zhang,
Receptor for detection of a Type II sex pheromone in the winter moth Operophtera brumata.
2016,
Pubmed
de Bruyne,
Odor coding in the Drosophila antenna.
2001,
Pubmed
