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XB-ART-55307
Mol Reprod Dev January 1, 2018; 85 (12): 896-908.

Combined functions of two RRMs in Dead-end1 mimic helicase activity to promote nanos1 translation in the germline.

Aguero T , Jin Z , Owens D , Malhotra A , Newman K , Yang J , King ML .


Abstract
Dead-end1 (Dnd1) expression is restricted to the vertebrate germline where it is believed to activate translation of messenger RNAs (mRNAs) required to protect and promote that unique lineage. Nanos1 is one such germline mRNA whose translation is blocked by a secondary mRNA structure within the open reading frame (ORF). Dnd1 contains a canonical RNA recognition motif (RRM1) in its N-terminus but also contains a less conserved RRM2. Here we provide a mechanistic picture of the nanos1 mRNA-Dnd1 interaction in the Xenopus germline. We show that RRM1, but not RRM2, is required for binding nanos1. Similar to the zebrafish homolog, Xenopus Dnd1 possesses ATPase activity. Surprisingly, this activity appears to be within the RRM2, different from the C-terminal region where it is found in zebrafish. More importantly, we show that RRM2 is required for nanos1 translation and germline survival. Further, Dnd1 functions as a homodimer and binds nanos1 mRNA just downstream of the secondary structure required for nanos1 repression. We propose a model in which the RRM1 is required to bind nanos1 mRNA while the RRM2 is required to promote translation through the action of ATPase. Dnd1 appears to use RRMs to mimic the function of helicases.

PubMed ID: 30230100
PMC ID: PMC6294668
Article link: Mol Reprod Dev
Grant support: [+]

Species referenced: Xenopus
Genes referenced: dnd1 myc nanos1 pgat pgc rrm1 rrm2.2 tbl1x tubb3
GO keywords: translation [+]
Antibodies: Dnd1 Ab1 Myc Ab12 Nanos1 Ab1 Tubb3 Ab2


Article Images: [+] show captions
References [+] :
Aguero, Microinjection of <i>Xenopus</i> Oocytes. 2018, Pubmed, Xenbase