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XB-ART-55322
Elife January 1, 2018; 7

Par3 interacts with Prickle3 to generate apical PCP complexes in the vertebrate neural plate.

Chuykin I , Ossipova O , Sokol SY .


Abstract
Vertebrate neural tube formation depends on the coordinated orientation of cells in the tissue known as planar cell polarity (PCP). In the Xenopus neural plate, PCP is marked by the enrichment of the conserved proteins Prickle3 and Vangl2 at anterior cell boundaries. Here we show that the apical determinant Par3 is also planar polarized in the neuroepithelium, suggesting a role for Par3 in PCP. Consistent with this hypothesis, interference with Par3 activity inhibited asymmetric distribution of PCP junctional complexes and caused neural tube defects. Importantly, Par3 physically associated with Prickle3 and promoted its apical localization, whereas overexpression of a Prickle3-binding Par3 fragment disrupted PCP in the neural plate. We also adapted proximity biotinylation assay for use in Xenopus embryos and show that Par3 functions by enhancing the formation of the anterior apical PCP complex. These findings describe a mechanistic link between the apical localization of PCP components and morphogenetic movements underlying neurulation.

PubMed ID: 30256191
Article link: Elife
Grant support: [+]
Genes referenced: ctnnb1 dvl2 myc pard3 pkm prickle3 prkci prkcz tjp1 vangl2
GO keywords: neural tube formation [+]
Antibodies: Ctnnb1 Ab7 Pard3 Ab1 Prkcz Ab1 Tjp1 Ab1 Tjp1 Ab2 Vangl2 Ab4
Morpholinos: pard3 MO3 pard3 MO4 vangl2 MO4

Disease Ontology terms: neural tube defect

Article Images: [+] show captions
References [+] :
Achilleos, PAR-3 mediates the initial clustering and apical localization of junction and polarity proteins during C. elegans intestinal epithelial cell polarization. 2010, Pubmed


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