Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-55348
Development January 1, 2018; 145 (20):

Fam46a regulates BMP-dependent pre-placodal ectoderm differentiation in Xenopus.

Watanabe T , Yamamoto T , Tsukano K , Hirano S , Horikawa A , Michiue T .


Abstract
The pre-placodal ectoderm (PPE) is a specialized ectodermal region which gives rise to the sensory organs and other systems. The PPE is induced from the neural plate border during neurulation, but the molecular mechanism of PPE formation is not fully understood. In this study, we examined the role of a newly identified PPE gene, Fam46a, during embryogenesis. Fam46a contains a nucleoside triphosphate transferase domain, but its function in early development was previously unclear. We show that Fam46a is expressed in the PPE in Xenopus embryos, and Fam46a knockdown induces abnormalities in the eye formation and the body color. At the neurula stage, Fam46a upregulates the expression of PPE genes and inhibits neural crest formation. We also show that Fam46a physically interacts with Smad1/Smad4 and positively regulates BMP signaling. From these results, we conclude that Fam46a is required for PPE formation via the positive regulation of BMP signaling. Our study provides a new mechanism of ectodermal patterning via cell-autonomous regulation of BMP signaling in the PPE.

PubMed ID: 30291163
Article link: Development

Genes referenced: bmp4 bmpr2 chrd.1 dnai1 eef1a1 eef1a2 eya1 foxd3 krt12.4 myc ncam1 npb odc1 pax2 pax3 pax6 pax8 rpe six1 six3 six6 smad1 smad2 smad4.1 smad4.2 snai1 snai2 sox2 sox3 tent5a ventx1.2 ventx2.2 wnt8a zic1
GO keywords: camera-type eye development [+]
Morpholinos: Tent5a MO1


Article Images: [+] show captions


Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556