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XB-ART-55362
Dev Dyn January 1, 2018; 247 (11): 1199-1210.

Identification of retinal homeobox (rax) gene-dependent genes by a microarray approach: The DNA endoglycosylase neil3 is a major downstream component of the rax genetic pathway.

Pan Y , Kelly LE , El-Hodiri HM .


Abstract
BACKGROUND: The retinal homeobox (rx/rax) gene is a transcription factor expressed in the developing eye field that is necessary for normal eye development. rax is necessary for retinal specification and stem cell development. The genetic program of early retinal development, including rax expression, can be induced in naïve ectoderm by activation of insulin-like growth factor (IGF) signaling. We have undertaken a microarray-based approach to identify rax-dependent IGF-induced genes. RESULTS: We identified 21 IGF-induced genes that exhibit at least a two-fold decrease in expression when rax expression is knocked down. Ten of these genes were expressed in the developing eye, eight were expressed in the ciliary marginal zone of the mature tadpole retina, and four could significantly rescue the rax knockdown phenotype. One of these, the nei endonuclease VIII-like 3 (neil3) gene, rescued the rax knockdown phenotype to a remarkable degree. We found that neil3 is necessary for normal retinal lamination and retinal neuron differentiation. CONCLUSIONS: We have identified neil3 as a component of the rax genetic pathway necessary for normal retinal progenitor cell development. neil3 is involved in the base excision DNA repair pathway, suggesting that this pathway is essential for normal rax-dependent progenitor cell development in the mature retina. Developmental Dynamics 247:1199-1210, 2018. © 2018 Wiley Periodicals, Inc.

PubMed ID: 30311321
PMC ID: PMC6246801
Article link: Dev Dyn
Grant support: [+]
Genes referenced: abcf2 bnip3 cbx5 cox4i1 e2f7 fam50a ins isl1 neil3 neurod1 pax6 rax rho sox11 XB5768883
GO keywords: eye development
Antibodies: Isl1/2 Ab1
Morpholinos: neil3 MO1 rax MO3


Article Images: [+] show captions
References [+] :
Amato, Retinal stem cells in vertebrates: parallels and divergences. 2005, Pubmed, Xenbase


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