XB-ART-55451Nat Commun January 1, 2018; 9 (1): 4668.
Multiciliated cells (MCCs) harbor dozens to hundreds of motile cilia, which generate hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation involves massive centriole production by poorly characterized structures called deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome stage MCCs are characterized by the expression of many cell cycle-related genes. We further investigated the uncharacterized vertebrate-specific cell division cycle 20B (CDC20B) gene, which hosts microRNA-449abc. We show that CDC20B protein associates to deuterosomes and is required for centriole release and subsequent cilia production in mouse and Xenopus MCCs. CDC20B interacts with PLK1, a kinase known to coordinate centriole disengagement with the protease Separase in mitotic cells. Strikingly, over-expression of Separase rescues centriole disengagement and cilia production in CDC20B-deficient MCCs. This work reveals the shaping of deuterosome-mediated centriole production in vertebrate MCCs, by adaptation of canonical and recently evolved cell cycle-related molecules.
PubMed ID: 30405130
Article link: Nat Commun
Genes referenced: ccnb1 cdc20 cdc20b cdk1 cep43 cetn1 deup1 foxj1 mcc mcidas mki67 myb nek2 pcnt plk1 plk4 spag5 tjp1 tp63 tuba4b
Morpholinos: cdc20b MO1 cdc20b MO2
References [+] :
Al Jord, Calibrated mitotic oscillator drives motile ciliogenesis. 2018, Pubmed