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Cell January 1, 2019; 176 (1-2): 167-181.e21.

The CMG Helicase Bypasses DNA-Protein Cross-Links to Facilitate Their Repair.

Sparks JL , Chistol G , Gao AO , Räschle M , Larsen NB , Mann M , Duxin JP , Walter JC .

Covalent DNA-protein cross-links (DPCs) impede replication fork progression and threaten genome integrity. Using Xenopus egg extracts, we previously showed that replication fork collision with DPCs causes their proteolysis, followed by translesion DNA synthesis. We show here that when DPC proteolysis is blocked, the replicative DNA helicase CMG (CDC45, MCM2-7, GINS), which travels on the leading strand template, bypasses an intact leading strand DPC. Single-molecule imaging reveals that GINS does not dissociate from CMG during bypass and that CMG slows dramatically after bypass, likely due to uncoupling from the stalled leading strand. The DNA helicase RTEL1 facilitates bypass, apparently by generating single-stranded DNA beyond the DPC. The absence of RTEL1 impairs DPC proteolysis, suggesting that CMG must bypass the DPC to enable proteolysis. Our results suggest a mechanism that prevents inadvertent CMG destruction by DPC proteases, and they reveal CMG''s remarkable capacity to overcome obstacles on its translocation strand.

PubMed ID: 30595447
PMC ID: PMC6475077
Article link: Cell
Grant support: [+]
Genes referenced: mcm2

References [+] :
Arias, Replication-dependent destruction of Cdt1 limits DNA replication to a single round per cell cycle in Xenopus egg extracts. 2005, Pubmed, Xenbase

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