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Elife January 1, 2019; 8

Anillin regulates epithelial cell mechanics by structuring the medial-apical actomyosin network.

Arnold TR , Shawky JH , Stephenson RE , Dinshaw KM , Higashi T , Huq F , Davidson LA , Miller AL .

Cellular forces sculpt organisms during development, while misregulation of cellular mechanics can promote disease. Here, we investigate how the actomyosin scaffold protein anillin contributes to epithelial mechanics in Xenopus laevis embryos. Increased mechanosensitive recruitment of vinculin to cell-cell junctions when anillin is overexpressed suggested that anillin promotes junctional tension. However, junctional laser ablation unexpectedly showed that junctions recoil faster when anillin is depleted and slower when anillin is overexpressed. Unifying these findings, we demonstrate that anillin regulates medial-apical actomyosin. Medial-apical laser ablation supports the conclusion that that tensile forces are stored across the apical surface of epithelial cells, and anillin promotes the tensile forces stored in this network. Finally, we show that anillin''s effects on cellular mechanics impact tissue-wide mechanics. These results reveal anillin as a key regulator of epithelial mechanics and lay the groundwork for future studies on how anillin may contribute to mechanical events in development and disease.

PubMed ID: 30702429
PMC ID: PMC6424563
Article link: Elife
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actb anln cdh1 shroom3 vcl
GO keywords: cell-cell junction [+]
Morpholinos: anln MO1

Disease Ontology terms: cancer

Article Images: [+] show captions
References [+] :
Amini, C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization. 2015, Pubmed