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XB-ART-55717
Dev Biol May 1, 2019; 449 (1): 1-13.

The Wnt inhibitor Dkk1 is required for maintaining the normal cardiac differentiation program in Xenopus laevis.

Guo Y , Dorn T , Kühl SJ , Linnemann A , Rothe M , Pfister AS , Vainio S , Laugwitz KL , Moretti A , Kühl M .


Abstract
Wnt proteins can activate different intracellular signaling pathways. These pathways need to be tightly regulated for proper cardiogenesis. The canonical Wnt/β-catenin inhibitor Dkk1 has been shown to be sufficient to trigger cardiogenesis in gain-of-function experiments performed in multiple model systems. Loss-of-function studies however did not reveal any fundamental function for Dkk1 during cardiogenesis. Using Xenopus laevis as a model we here show for the first time that Dkk1 is required for proper differentiation of cardiomyocytes, whereas specification of cardiomyocytes remains unaffected in absence of Dkk1. This effect is at least in part mediated through regulation of non-canonical Wnt signaling via Wnt11. In line with these observations we also found that Isl1, a critical regulator for specification of the common cardiac progenitor cell (CPC) population, acts upstream of Dkk1.

PubMed ID: 30797757
PMC ID: PMC6496975
Article link: Dev Biol

Genes referenced: alcam bmp4 dkk1 frzb2 gata6 h3-3a hprt1 isl1 myh6 nkx2-5 tbx1 tbx5 tbxt tnni3 tnnt2 wnt11
GO keywords: Wnt signaling pathway [+]
Antibodies: Tnnt2 Ab1
Morpholinos: dkk1 MO1 isl1 MO1


Article Images: [+] show captions


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