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J Cell Biol January 1, 2018; 217 (3): 849-859.

An interaction between myosin-10 and the cell cycle regulator Wee1 links spindle dynamics to mitotic progression in epithelia.

Sandquist JC , Larson ME , Woolner S , Ding Z , Bement WM .

Anaphase in epithelia typically does not ensue until after spindles have achieved a characteristic position and orientation, but how or even if cells link spindle position to anaphase onset is unknown. Here, we show that myosin-10 (Myo10), a motor protein involved in epithelial spindle dynamics, binds to Wee1, a conserved regulator of cyclin-dependent kinase 1 (Cdk1). Wee1 inhibition accelerates progression through metaphase and disrupts normal spindle dynamics, whereas perturbing Myo10 function delays anaphase onset in a Wee1-dependent manner. Moreover, Myo10 perturbation increases Wee1-mediated inhibitory phosphorylation on Cdk1, which, unexpectedly, concentrates at cell-cell junctions. Based on these and other results, we propose a model in which the Myo10-Wee1 interaction coordinates attainment of spindle position and orientation with anaphase onset.

PubMed ID: 29321170
Article link: J Cell Biol
Grant support: [+]
Genes referenced: cdc25a cdk1 h2bc21 myo10 wee1 wee2
GO keywords: spindle [+]
Morpholinos: myo10.2 MO5

Article Images: [+] show captions
References [+] :
Adams, Metaphase spindles rotate in the neuroepithelium of rat cerebral cortex. 1997, Pubmed

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