XB-ART-55931
J Phys Chem B
2019 May 30;12321:4527-4533. doi: 10.1021/acs.jpcb.9b02162.
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Positively Charged Tags Impede Protein Mobility in Cells as Quantified by 19F NMR.
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Proteins are often tagged for visualization or delivery in the "sea" of other macromolecules in cells but how tags affect protein mobility remains poorly understood. Here, we employ in-cell 19F NMR to quantify the mobility of proteins with charged tags in Escherichia coli cells and Xenopus laevis oocytes. We find that the transient charge-charge interactions between the tag and cellular components affect protein mobility. More specifically, positively charged tags impede protein mobility.
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