Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Dokl Biochem Biophys
2019 May 01;4841:9-12. doi: 10.1134/S1607672919010034.
Show Gene links
Show Anatomy links
Recombinant Production and Structure-Function Study of the Ts1 Toxin from the Brazilian Scorpion Tityus serrulatus.
Shenkarev ZO
,
Shulepko MA
,
Peigneur S
,
Myshkin MY
,
Berkut AA
,
Vassilevski AA
,
Tytgat J
,
Lyukmanova EN
,
Kirpichnikov MP
.
???displayArticle.abstract???
An effective bacterial system for the production of β-toxin Ts1, the main component of the Brazilian scorpion Tityus serrulatus venom, was developed. Recombinant toxin and its 15N-labeled analogue were obtained via direct expression of synthetic gene in Escherichia coli with subsequent folding from the inclusion bodies. According to NMR spectroscopy data, the recombinant toxin is structured in an aqueous solution and contains a significant fraction of β-structure. The formation of a stable disulfide-bond isomer of Ts1, having a disordered structure, has also been observed during folding. Recombinant Ts1 blocks Na+ current through NaV1.5 channels without affecting the processes of activation and inactivation. At the same time, the effect upon NaV1.4 channels is associated with a shift of the activation curve towards more negative membrane potentials.
Barhanin,
Tityus serrulatus venom contains two classes of toxins. Tityus gamma toxin is a new tool with a very high affinity for studying the Na+ channel.
1982, Pubmed
Barhanin,
Tityus serrulatus venom contains two classes of toxins. Tityus gamma toxin is a new tool with a very high affinity for studying the Na+ channel.
1982,
Pubmed
Bosmans,
Differential effects of five 'classical' scorpion beta-toxins on rNav1.2a and DmNav1 provide clues on species-selectivity.
2007,
Pubmed
,
Xenbase
Bosmans,
Deconstructing voltage sensor function and pharmacology in sodium channels.
2008,
Pubmed
,
Xenbase
Catterall,
Voltage-gated sodium channels at 60: structure, function and pathophysiology.
2012,
Pubmed
Coelho,
Functional and structural study comparing the C-terminal amidated β-neurotoxin Ts1 with its isoform Ts1-G isolated from Tityus serrulatus venom.
2014,
Pubmed
Dang,
Total chemical synthesis of biologically active fluorescent dye-labeled Ts1 toxin.
2014,
Pubmed
Loret,
Conformational flexibility of a scorpion toxin active on mammals and insects: a circular dichroism study.
1990,
Pubmed
Martin-Eauclaire,
Ts1 from the Brazilian scorpion Tityus serrulatus: A half-century of studies on a multifunctional beta like-toxin.
2018,
Pubmed
Männikkö,
Spider toxin inhibits gating pore currents underlying periodic paralysis.
2018,
Pubmed
,
Xenbase
Peigneur,
A gamut of undiscovered electrophysiological effects produced by Tityus serrulatus toxin 1 on NaV-type isoforms.
2015,
Pubmed
,
Xenbase
Pinheiro,
Structural analysis of Tityus serrulatus Ts1 neurotoxin at atomic resolution: insights into interactions with Na+ channels.
2003,
Pubmed
Pluzhnikov,
omega-Lsp-IA, a novel modulator of P-type Ca2+ channels.
2007,
Pubmed
Shulepko,
Towards universal approach for bacterial production of three-finger Ly6/uPAR proteins: Case study of cytotoxin I from cobra N. oxiana.
2017,
Pubmed
Stevens,
Neurotoxins and their binding areas on voltage-gated sodium channels.
2011,
Pubmed
