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XB-ART-55940
Nat Commun January 1, 2019; 10 (1): 1673.

Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity.

Chakraborty M , Tarasovetc EV , Zaytsev AV , Godzi M , Figueiredo AC , Ataullakhanov FI , Grishchuk EL .


Abstract
Accurate chromosome segregation relies on microtubule end conversion, the ill-understood ability of kinetochores to transit from lateral microtubule attachment to durable association with dynamic microtubule plus-ends. The molecular requirements for this conversion and the underlying biophysical mechanisms are elusive. We reconstituted end conversion in vitro using two kinetochore components: the plus end-directed kinesin CENP-E and microtubule-binding Ndc80 complex, combined on the surface of a microbead. The primary role of CENP-E is to ensure close proximity between Ndc80 complexes and the microtubule plus-end, whereas Ndc80 complexes provide lasting microtubule association by diffusing on the microtubule wall near its tip. Together, these proteins mediate robust plus-end coupling during several rounds of microtubule dynamics, in the absence of any specialized tip-binding or regulatory proteins. Using a Brownian dynamics model, we show that end conversion is an emergent property of multimolecular ensembles of microtubule wall-binding proteins with finely tuned force-dependent motility characteristics.

PubMed ID: 30975984
Article link: Nat Commun
Grant support: [+]
Genes referenced: ndc80



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