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Mol Biol Cell January 1, 2019; 30 (16): 1900-1910.

DAPLE and MPDZ bind to each other and cooperate to promote apical cell constriction.

Marivin A , Garcia-Marcos M .

O-1) domain scaffold present at apical cell junctions whose mutation in humans is linked to nonsyndromic congenital hydrocephalus (NSCH). DAPLE contains a PDZ-binding motif (PBM) and is also mutated in human NSCH, so we investigated the functional relationship between both proteins. DAPLE colocalized with MPDZ at apical cell junctions and bound directly to the PDZ3 domain of MPDZ via its PBM. Much like DAPLE, MPDZ is induced during neurulation in Xenopus and is required for apical constriction of neuroepithelial cells and subsequent neural plate bending. MPDZ depletion also blunted DAPLE--mediated apical constriction of cultured cells. These results show that DAPLE and MPDZ, two factors genetically linked to NSCH, function as cooperative partners at apical junctions and are required for proper tissue remodeling during early stages of neurodevelopment.

PubMed ID: 31268831
PMC ID: PMC6727773
Article link: Mol Biol Cell
Grant support: [+]
Genes referenced: ccdc88c ctnnb1 isyna1 myc patj

Article Images: [+] show captions
References [+] :
Adachi, Similar and distinct properties of MUPP1 and Patj, two homologous PDZ domain-containing tight-junction proteins. 2009, Pubmed

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