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XB-ART-56249
PLoS One January 1, 2019; 14 (8): e0221698.

Divergent roles of the Wnt/PCP Formin Daam1 in renal ciliogenesis.

Corkins ME , Krneta-Stankic V , Kloc M , McCrea PD , Gladden AB , Miller RK .


Abstract
Kidneys are composed of numerous ciliated epithelial tubules called nephrons. Each nephron functions to reabsorb nutrients and concentrate waste products into urine. Defects in primary cilia are associated with abnormal formation of nephrons and cyst formation in a wide range of kidney disorders. Previous work in Xenopus laevis and zebrafish embryos established that loss of components that make up the Wnt/PCP pathway, Daam1 and ArhGEF19 (wGEF) perturb kidney tubulogenesis. Dishevelled, which activates both the canonical and non-canonical Wnt/PCP pathway, affect cilia formation in multiciliated cells. In this study, we investigated the role of the noncanoncial Wnt/PCP components Daam1 and ArhGEF19 (wGEF) in renal ciliogenesis utilizing polarized mammalian kidney epithelia cells (MDCKII and IMCD3) and Xenopus laevis embryonic kidney. We demonstrate that knockdown of Daam1 and ArhGEF19 in MDCKII and IMCD3 cells leads to loss of cilia, and Daam1''s effect on ciliogenesis is mediated by the formin-activity of Daam1. Moreover, Daam1 co-localizes with the ciliary transport protein Ift88 and is present in cilia. Interestingly, knocking down Daam1 in Xenopus kidney does not lead to loss of cilia. These data suggests a new role for Daam1 in the formation of primary cilia.

PubMed ID: 31469868
PMC ID: PMC6716777
Article link: PLoS One
Grant support: [+]
Genes referenced: cby1 daam1 dvl2 fmn1 grap2 ift88 lhx1 ran
GO keywords: Wnt signaling pathway

Disease Ontology terms: Joubert syndrome [+]

Article Images: [+] show captions
References [+] :
Alberts, Identification of a carboxyl-terminal diaphanous-related formin homology protein autoregulatory domain. 2001, Pubmed


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