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XB-ART-566
Nat Cell Biol. April 1, 2006; 8 (4): 348-57.

The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation.

Angers S , Thorpe CJ , Biechele TL , Goldenberg SJ , Zheng N , MacCoss MJ , Moon RT .


Abstract
Dishevelled is a conserved protein that interprets signals received by Frizzled receptors. Using a tandem-affinity purification strategy and mass spectrometry we have identified proteins associated with Dishevelled, including a Cullin-3 ubiquitin ligase complex containing the Broad Complex, Tramtrack and Bric à Brac (BTB) protein Kelch-like 12 (KLHL12). This E3 ubiquitin ligase complex is recruited to Dishevelled in a Wnt-dependent manner that promotes its poly-ubiquitination and degradation. Functional analyses demonstrate that regulation of Dishevelled by this ubiquitin ligase antagonizes the Wnt-beta-catenin pathway in cultured cells, as well as in Xenopus and zebrafish embryos. Considered with evidence that the distinct Cullin-1 based SCF(beta-TrCP)complex regulates beta-catenin stability, our data on the stability of Dishevelled demonstrates that two distinct ubiquitin ligase complexes regulate the Wnt-beta-catenin pathway.

PubMed ID: 16547521
Article link: Nat Cell Biol.
Grant support: P41 RR011823 NCRR NIH HHS

Genes referenced: atp8b1 btrc cul1 dvl2 klhl12
Antibodies referenced:
Morpholinos referenced:

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