Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
AtDUR3 encodes a new type of high-affinity urea/H+ symporter in Arabidopsis.
Liu LH
,
Ludewig U
,
Frommer WB
,
von Wirén N
.
???displayArticle.abstract???
Urea is the major nitrogen form supplied as fertilizer in agricultural plant production but also an important nitrogen metabolite in plants. We report the cloning and functional characterization of AtDUR3, a high-affinity urea transporter in plants. AtDUR3 contains 14 putative transmembrane-spanning domains and represents an individual member in Arabidopsis that belongs to a superfamily of sodium-solute symporters. Heterologous expression in urea uptake-defective yeast as well as two-electrode voltage clamp and uptake studies using (14)C-labeled urea in AtDUR3-expressing oocytes demonstrated that AtDUR3 mediates urea transport. In both heterologous systems, urea transport was stimulated at low pH. In oocytes, inward currents indicated that urea is cotransported with protons. By contrast, a supply of Na(+) ions could not stimulate urea transport. Transport of (14)C-labeled urea by AtDUR3 in oocytes exhibited saturation kinetics with a K(m) of approximately 3 micro M. AtDUR3 was expressed in shoots and roots and upregulated during early germination and under nitrogen deficiency in roots. We propose a role of AtDUR3 in urea uptake by plant cells at low external urea concentrations.
An,
Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues.
1996, Pubmed
An,
Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues.
1996,
Pubmed
Desimone,
A novel superfamily of transporters for allantoin and other oxo derivatives of nitrogen heterocyclic compounds in Arabidopsis.
2002,
Pubmed
,
Xenbase
Dohmen,
An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera.
1991,
Pubmed
Echevarría,
Aquaporins.
1998,
Pubmed
ElBerry,
Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae.
1993,
Pubmed
Gazzarrini,
Three functional transporters for constitutive, diurnally regulated, and starvation-induced uptake of ammonium into Arabidopsis roots.
1999,
Pubmed
Gerbeau,
Aquaporin Nt-TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes.
1999,
Pubmed
,
Xenbase
Grenson,
The utilization of exogenous pyrimidines and the recycling of uridine-5'-phosphate derivatives in Saccharomyces cerevisiae, as studied by means of mutants affected in pyrimidine uptake and metabolism.
1969,
Pubmed
Güldener,
A new efficient gene disruption cassette for repeated use in budding yeast.
1996,
Pubmed
Hinderhofer,
Identification of a transcription factor specifically expressed at the onset of leaf senescence.
2001,
Pubmed
Krogmeier,
Phytotoxicity of foliar-applied urea.
1989,
Pubmed
Lejay,
Molecular and functional regulation of two NO3- uptake systems by N- and C-status of Arabidopsis plants.
1999,
Pubmed
Leung,
Urea transport by cotransporters.
2000,
Pubmed
,
Xenbase
Ludewig,
Uniport of NH4+ by the root hair plasma membrane ammonium transporter LeAMT1;1.
2002,
Pubmed
,
Xenbase
Masclaux,
Characterization of the sink/source transition in tobacco ( Nicotiana tabacum L.) shoots in relation to nitrogen management and leaf senescence.
2000,
Pubmed
May,
Oxidative Stimulation of Glutathione Synthesis in Arabidopsis thaliana Suspension Cultures.
1993,
Pubmed
Minet,
Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs.
1992,
Pubmed
Oliveira,
Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis.
1999,
Pubmed
Rawat,
AtAMT1 gene expression and NH4+ uptake in roots of Arabidopsis thaliana: evidence for regulation by root glutamine levels.
1999,
Pubmed
Reizer,
A functional superfamily of sodium/solute symporters.
1994,
Pubmed
Saier,
A functional-phylogenetic classification system for transmembrane solute transporters.
2000,
Pubmed
Saraste,
Structural features of cytochrome oxidase.
1990,
Pubmed
Shayakul,
Molecular cloning and characterization of the vasopressin-regulated urea transporter of rat kidney collecting ducts.
1996,
Pubmed
,
Xenbase
Smith,
Facilitative urea transporters.
2001,
Pubmed
Sonnhammer,
A hidden Markov model for predicting transmembrane helices in protein sequences.
1998,
Pubmed
Turk,
Membrane topology motifs in the SGLT cotransporter family.
1997,
Pubmed
Valladares,
An ABC-type, high-affinity urea permease identified in cyanobacteria.
2002,
Pubmed
Walker,
The uptake and metabolism of urea by Chara australis: IV. Symport with sodium--a slip model for the high and low affinity systems.
1993,
Pubmed
Weeks,
A H+-gated urea channel: the link between Helicobacter pylori urease and gastric colonization.
2000,
Pubmed
,
Xenbase
Wieczorke,
Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae.
1999,
Pubmed
Witte,
Leaf urea metabolism in potato. Urease activity profile and patterns of recovery and distribution of (15)N after foliar urea application in wild-type and urease-antisense transgenics.
2002,
Pubmed
Zonia,
Essential role of urease in germination of nitrogen-limited Arabidopsis thaliana seeds.
1995,
Pubmed
