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J Cell Biol April 6, 2020; 219 (4):

A direct role for SNX9 in the biogenesis of filopodia.

Jarsch IK , Gadsby JR , Nuccitelli A , Mason J , Shimo H , Pilloux L , Marzook B , Mulvey CM , Dobramysl U , Bradshaw CR , Lilley KS , Hayward RD , Vaughan TJ , Dobson CL , Gallop JL .

Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell-cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia.

PubMed ID: 32328641
Article link: J Cell Biol
Grant support: [+]
Genes referenced: pik3ca pmch rpe snx9 vasp
GO keywords: filopodium

Article Images: [+] show captions
References [+] :
Bannister, Parallel, high-throughput purification of recombinant antibodies for in vivo cell assays. 2006, Pubmed

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