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XB-ART-56926
J Cell Biol April 6, 2020; 219 (4):

FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells.

Smith JA , Curry EG , Blue RE , Roden C , Dundon SER , Rodríguez-Vargas A , Jordan DC , Chen X , Lyons SM , Crutchley J , Anderson P , Horb ME , Gladfelter AS , Giudice J .


Abstract
Fragile-X mental retardation autosomal homologue-1 (FXR1) is a muscle-enriched RNA-binding protein. FXR1 depletion is perinatally lethal in mice, Xenopus, and zebrafish; however, the mechanisms driving these phenotypes remain unclear. The FXR1 gene undergoes alternative splicing, producing multiple protein isoforms and mis-splicing has been implicated in disease. Furthermore, mutations that cause frameshifts in muscle-specific isoforms result in congenital multi-minicore myopathy. We observed that FXR1 alternative splicing is pronounced in the serine- and arginine-rich intrinsically disordered domain; these domains are known to promote biomolecular condensation. Here, we show that tissue-specific splicing of fxr1 is required for Xenopus development and alters the disordered domain of FXR1. FXR1 isoforms vary in the formation of RNA-dependent biomolecular condensates in cells and in vitro. This work shows that regulation of tissue-specific splicing can influence FXR1 condensates in muscle development and how mis-splicing promotes disease.

PubMed ID: 32328638
PMC ID: PMC7147106
Article link: J Cell Biol
Grant support: [+]
Genes referenced: fxr1

References [+] :
Adams-Cioaba, Structural studies of the tandem Tudor domains of fragile X mental retardation related proteins FXR1 and FXR2. 2011, Pubmed


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