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XB-ART-56933
Elife April 27, 2020; 9

Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors.

Kakebeen AD , Chitsazan AD , Williams MC , Saunders LM , Wills AE .


Abstract
Vertebrate appendage regeneration requires precisely coordinated remodeling of the transcriptional landscape to enable the growth and differentiation of new tissue, a process executed over multiple days and across dozens of cell types. The heterogeneity of tissues and temporally-sensitive fate decisions involved has made it difficult to articulate the gene regulatory programs enabling regeneration of individual cell types. To better understand how a regenerative program is fulfilled by neural progenitor cells (NPCs) of the spinal cord, we analyzed pax6-expressing NPCs isolated from regenerating Xenopus tropicalis tails. By intersecting chromatin accessibility data with single-cell transcriptomics, we find that NPCs place an early priority on neuronal differentiation. Late in regeneration, the priority returns to proliferation. Our analyses identify Pbx3 and Meis1 as critical regulators of tail regeneration and axon organization. Overall, we use transcriptional regulatory dynamics to present a new model for cell fate decisions and their regulators in NPCs during regeneration.

PubMed ID: 32338593
PMC ID: PMC7250574
Article link: Elife
Grant support: [+]
Genes referenced: actc1 dcx dnai1 elavl4 l1cam meis1 neurod1 neurog1 odc1 pafah1b1 pax6 sox2 tubb2b uchl1
GO keywords: regeneration

GEO Series: GSE146830: NCBI
GSE146836: NCBI

Article Images: [+] show captions
References [+] :
Alaynick, SnapShot: spinal cord development. 2011, Pubmed


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