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XB-ART-56942
Int J Mol Sci April 26, 2020; 21 (9):

Effects of Ferrocenyl 4-(Imino)-1,4-Dihydro-quinolines on Xenopus laevis Prophase I - Arrested Oocytes: Survival and Hormonal-Induced M-Phase Entry.

Marchand G , Wambang N , Pellegrini S , Molinaro C , Martoriati A , Bousquet T , Markey A , Lescuyer-Rousseau A , Bodart JF , Cailliau K , Pelinski L , Marin M .


Abstract
Xenopus oocytes were used as cellular and molecular sentinels to assess the effects of a new class of organometallic compounds called ferrocenyl dihydroquinolines that have been developed as potential anti-cancer agents. One ferrocenyl dihydroquinoline compound exerted deleterious effects on oocyte survival after 48 h of incubation at 100 μM. Two ferrocenyl dihydroquinoline compounds had an inhibitory effect on the resumption of progesterone induced oocyte meiosis, compared to controls without ferrocenyl groups. In these inhibited oocytes, no MPF (Cdk1/cyclin B) activity was detected by western blot analysis as shown by the lack of phosphorylation of histone H3. The dephosphorylation of the inhibitory Y15 residue of Cdk1 occurred but cyclin B was degraded. Moreover, two apoptotic death markers, the active caspase 3 and the phosphorylated histone H2, were detected. Only 7-chloro-1-ferrocenylmethyl-4-(phenylylimino)-1,4-dihydroquinoline (8) did not show any toxicity and allowed the assembly of a histologically normal metaphase II meiotic spindle while inhibiting the proliferation of cancer cell lines with a low IC50, suggesting that this compound appears suitable as an antimitotic agent.

PubMed ID: 32357477
PMC ID: PMC7246863
Article link: Int J Mol Sci

Genes referenced: cdk1 h2bc21 mapk1 mos myt1 psmd6 rasgrf1 rps6ka1
GO keywords: meiotic metaphase II [+]


Article Images: [+] show captions


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