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XB-ART-56979
Elife January 1, 2020; 9

Cohesin and condensin extrude DNA loops in a cell cycle-dependent manner.

Golfier S , Quail T , Kimura H , Brugués J .


Abstract
Loop extrusion by structural maintenance of chromosomes (SMC) complexes has been proposed as a mechanism to organize chromatin in interphase and metaphase. However, the requirements for chromatin organization in these cell cycle phases are different, and it is unknown whether loop extrusion dynamics and the complexes that extrude DNA also differ. Here, we used Xenopus egg extracts to reconstitute and image loop extrusion of single DNA molecules during the cell cycle. We show that loops form in both metaphase and interphase, but with distinct dynamic properties. Condensin extrudes DNA loops non-symmetrically in metaphase, whereas cohesin extrudes loops symmetrically in interphase. Our data show that loop extrusion is a general mechanism underlying DNA organization, with dynamic and structural properties that are biochemically regulated during the cell cycle.

PubMed ID: 32396063
PMC ID: PMC7316503
Article link: Elife
Grant support: [+]

Species referenced: Xenopus
Genes referenced: kcnt1 rad21 smc1a smc1b


Article Images: [+] show captions
References [+] :
Abramo, A chromosome folding intermediate at the condensin-to-cohesin transition during telophase. 2020, Pubmed