Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Front Physiol
2020 Jan 01;11:413. doi: 10.3389/fphys.2020.00413.
Show Gene links
Show Anatomy links
An Orphan Pheromone Receptor Affects the Mating Behavior of Helicoverpa armigera.
Cao S
,
Huang T
,
Shen J
,
Liu Y
,
Wang G
.
???displayArticle.abstract???
The Lepidoptera is the second largest insect order, which has the most extensive knowledge of sex pheromones and mechanisms of pheromone communication since the identification of the first insect pheromone in Bombyx mori. In the past 15 years, pheromone receptors have been identified and functionally characterized in many moth species. HarmOR14 is a typical pheromone receptor of Helicoverpa armigera which showed no response to the tested pheromones in Xenopus oocyte expression system, but its orthologous gene in Heliothis virescens, HvirOR14 could be activated by pheromones in the same expression system. To assess the possible functions of OR14 in vivo, in this study, we knocked out this gene using CRISPR/Cas9 system and compared the mating behaviors and EAG response to pheromones between the wild type and mutant strains. Our results showed that OR14 mutants did not affect the mating rate or the EAG response to pheromones but could prolong the mating duration and change the mating time in undefined manners, which extends our understanding to this kind of pheromone receptors.
FIGURE 1. Genetic mapping of pheromone receptors in H. armigera genome. Five PR genes including OR14b, OR14, OR15, OR16, and OR6 are located in a same scaffold (Harm_1.0 scaffold_53, NW_018395443.1) in tandem arrays. The visible map was generated using the tool GSDS2.0 (http://gsds.cbi.pku.edu.cn) according to the loci information of PR genes.
FIGURE 2. CRISPR/Cas9-based knock out of H. armigera OR14. (A) The HarmOR14 protein possesses seven transmembrane domains. The seven transmembrane domains were predicted on TOPCONS website (http://topcons.net/). The nucleotide deletion was generated in the first transmembrane domain (green circle). (B) The HarmOR14 gene contains nine exons in genome and the exon 2 was targeted for CRISPR single-guide RNA (sgRNA) design (red region). PAM site was indicated in purple and the deletion was indicated as dash lines. The mutation caused a frameshift at codon 75 and a premature stop codon, giving rise to a truncated protein of 80 amino acids. (C) The three sequencing chromatograms indicated three genotypes, WT (top), heterozygote (middle) and homozygote (bottom), respectively, and the mutation site was shown with a red frame.
FIGURE 3. The mating behavior of wild type and OR14 mutant strains. (A) Schematic representation of behavioral experiment design. (B) Scatter-dot plot comparing the mating rate of wild type (left, red) and OR14 mutant (right, green) strains. Each dot represents the mating rate of one group. Error bars indicate SEM. n = 22 and 13, respectively. Student’s t-test, df = 33, t = –0.05, P = 0.962. (C) Scatter-dot plot comparing the mating duration of wild type (left, red) and OR14 mutant (right, green) strains. Each dot represents the mating duration of one pair of successful mating moths. n = 96 and 102, respectively. Student’s t-test, df = 184, t = 6.23, P < 0.001. n.s., no significant difference; *P < 0.05; **P < 0.01; ***P < 0.001. (D) Line graph showing the mating rate in each hour from 20:00 to 06:00 of wild type (red) and OR14 mutant (green) strains.
FIGURE 4. EAG responses of wild type and OR14 knockout males to pheromone blends in different concentrations. (A–C) Dose-response curves of wild type (green) and OR14 mutated male (red) antennae stimulated with Z11-16:Ald, Z9-16:Ald and Z9-14:Ald, respectively. Error bars indicate SEM. n = 18–20. *P < 0.05; **P < 0.01; ***P < 0.001.
Baker,
Balanced olfactory antagonism as a concept for understanding evolutionary shifts in moth sex pheromone blends.
2008, Pubmed
Baker,
Balanced olfactory antagonism as a concept for understanding evolutionary shifts in moth sex pheromone blends.
2008,
Pubmed
Bengtsson,
Putative chemosensory receptors of the codling moth, Cydia pomonella, identified by antennal transcriptome analysis.
2012,
Pubmed
Cabrera,
Sex pheromone of tomato fruit borer, Neoleucinodes elegantalis.
2001,
Pubmed
Cao,
A Conserved Odorant Receptor Tuned to Floral Volatiles in Three Heliothinae Species.
2016,
Pubmed
,
Xenbase
Cattaneo,
Candidate pheromone receptors of codling moth Cydia pomonella respond to pheromones and kairomones.
2017,
Pubmed
Chang,
Pheromone binding proteins enhance the sensitivity of olfactory receptors to sex pheromones in Chilo suppressalis.
2015,
Pubmed
,
Xenbase
Chang,
Sensillar expression and responses of olfactory receptors reveal different peripheral coding in two Helicoverpa species using the same pheromone components.
2016,
Pubmed
Chang,
A Pheromone Antagonist Regulates Optimal Mating Time in the Moth Helicoverpa armigera.
2017,
Pubmed
Cheng,
Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest.
2017,
Pubmed
Durand,
Degradation of pheromone and plant volatile components by a same odorant-degrading enzyme in the cotton leafworm, Spodoptera littoralis.
2011,
Pubmed
Engsontia,
Molecular evolution of the odorant and gustatory receptor genes in lepidopteran insects: implications for their adaptation and speciation.
2014,
Pubmed
Forstner,
A receptor and binding protein interplay in the detection of a distinct pheromone component in the silkmoth Antheraea polyphemus.
2009,
Pubmed
Garczynski,
CRISPR/Cas9 Editing of the Codling Moth (Lepidoptera: Tortricidae) CpomOR1 Gene Affects Egg Production and Viability.
2017,
Pubmed
Gibb,
(Z)-11-Hexadecenal and (3Z,6Z,9Z)-tricosatriene: sex pheromone components of the red banded mango caterpillar Deanolis sublimbalis.
2007,
Pubmed
Gonzalez,
Antennal transcriptomes of three tortricid moths reveal putative conserved chemosensory receptors for social and habitat olfactory cues.
2017,
Pubmed
Gould,
Sexual isolation of male moths explained by a single pheromone response QTL containing four receptor genes.
2010,
Pubmed
Grosse-Wilde,
Antennal transcriptome of Manduca sexta.
2011,
Pubmed
Hou,
Functional characterization of odorant receptors from the moth Eriocrania semipurpurella: A comparison of results in the Xenopus oocyte and HEK cell systems.
2020,
Pubmed
,
Xenbase
International Silkworm Genome Consortium,
The genome of a lepidopteran model insect, the silkworm Bombyx mori.
2008,
Pubmed
Ishida,
Rapid inactivation of a moth pheromone.
2005,
Pubmed
Jacquin-Joly,
Candidate chemosensory genes in female antennae of the noctuid moth Spodoptera littoralis.
2012,
Pubmed
Jordan,
Odorant receptors from the light brown apple moth (Epiphyas postvittana) recognize important volatile compounds produced by plants.
2009,
Pubmed
Kanost,
Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta.
2016,
Pubmed
Krieger,
Genes encoding candidate pheromone receptors in a moth (Heliothis virescens).
2004,
Pubmed
Krieger,
Candidate pheromone receptors of the silkmoth Bombyx mori.
2005,
Pubmed
Larsson,
Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction.
2004,
Pubmed
Linn,
Pheromone components and active spaces: what do moths smell and where do they smell it?
1987,
Pubmed
Liu,
Identification and characterization of three chemosensory receptor families in the cotton bollworm Helicoverpa armigera.
2014,
Pubmed
Liu,
Candidate olfaction genes identified within the Helicoverpa armigera Antennal Transcriptome.
2012,
Pubmed
Liu,
Identification and functional characterization of sex pheromone receptors in beet armyworm Spodoptera exigua (Hübner).
2013,
Pubmed
,
Xenbase
Liu,
Functional specificity of sex pheromone receptors in the cotton bollworm Helicoverpa armigera.
2013,
Pubmed
Millar,
(3Z,6Z,9Z, 12Z, 15Z)-Pentacosapentaene, a key pheromone component of the fir coneworm moth, Dioryctria abietivorella.
2005,
Pubmed
Miura,
Broadly and narrowly tuned odorant receptors are involved in female sex pheromone reception in Ostrinia moths.
2010,
Pubmed
Montagné,
Functional characterization of a sex pheromone receptor in the pest moth Spodoptera littoralis by heterologous expression in Drosophila.
2012,
Pubmed
Nakagawa,
Insect sex-pheromone signals mediated by specific combinations of olfactory receptors.
2005,
Pubmed
,
Xenbase
Pearce,
Genomic innovations, transcriptional plasticity and gene loss underlying the evolution and divergence of two highly polyphagous and invasive Helicoverpa pest species.
2017,
Pubmed
Pregitzer,
The sensory neurone membrane protein SNMP1 contributes to the sensitivity of a pheromone detection system.
2014,
Pubmed
Sakurai,
Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori.
2004,
Pubmed
,
Xenbase
Sakurai,
Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth.
2015,
Pubmed
Sato,
Insect olfactory receptors are heteromeric ligand-gated ion channels.
2008,
Pubmed
,
Xenbase
Steinwender,
Pheromone receptor evolution in the cryptic leafroller species, Ctenopseustis obliquana and C. herana.
2015,
Pubmed
Steinwender,
Odorant Receptors of the New Zealand Endemic Leafroller Moth Species Planotortrix octo and P. excessana.
2016,
Pubmed
Su,
Non-synaptic inhibition between grouped neurons in an olfactory circuit.
2012,
Pubmed
Teal,
Identification of a sex pheromone ofHeliothis subflexa (GN.) (Lepidoptera: Noctuidae) and field trapping studies using different blends of components.
1981,
Pubmed
Walker,
Transcriptome Analysis of Gene Families Involved in Chemosensory Function in Spodoptera littoralis (Lepidoptera: Noctuidae).
2019,
Pubmed
Wang,
Comparison of research methods for functional characterization of insect olfactory receptors.
2016,
Pubmed
Wang,
Functional characterization of pheromone receptors in the tobacco budworm Heliothis virescens.
2011,
Pubmed
Wicher,
Identification and characterization of the bombykal receptor in the hawkmoth Manduca sexta.
2017,
Pubmed
,
Xenbase
Wu,
Specific olfactory neurons and glomeruli are associated to differences in behavioral responses to pheromone components between two Helicoverpa species.
2015,
Pubmed
Xu,
Chemosensory receptor genes in the Oriental tobacco budworm Helicoverpa assulta.
2015,
Pubmed
Yang,
Two single-point mutations shift the ligand selectivity of a pheromone receptor between two closely related moth species.
2017,
Pubmed
,
Xenbase
You,
A heterozygous moth genome provides insights into herbivory and detoxification.
2013,
Pubmed
Yuvaraj,
Functional characterization of odorant receptors from Lampronia capitella suggests a non-ditrysian origin of the lepidopteran pheromone receptor clade.
2018,
Pubmed
Yuvaraj,
Characterization of Odorant Receptors from a Non-ditrysian Moth, Eriocrania semipurpurella Sheds Light on the Origin of Sex Pheromone Receptors in Lepidoptera.
2017,
Pubmed
Yuvaraj,
Antennal Transcriptome Analysis of the Chemosensory Gene Families From Trichoptera and Basal Lepidoptera.
2018,
Pubmed
Zhang,
Sequencing and characterization of six cDNAs putatively encoding three pairs of pheromone receptors in two sibling species, Helicoverpa armigera and Helicoverpa assulta.
2010,
Pubmed
Zhang,
Asymmetric ephaptic inhibition between compartmentalized olfactory receptor neurons.
2019,
Pubmed
Zhang,
An overlooked component: (Z)-9-tetradecenal as a sex pheromone in Helicoverpa armigera.
2012,
Pubmed
Zhang,
Receptor for detection of a Type II sex pheromone in the winter moth Operophtera brumata.
2016,
Pubmed
Zhang,
Antennal transcriptome analysis and comparison of chemosensory gene families in two closely related noctuidae moths, Helicoverpa armigera and H. assulta.
2015,
Pubmed
Zhang,
Functional evolution of a multigene family: orthologous and paralogous pheromone receptor genes in the turnip moth, Agrotis segetum.
2013,
Pubmed
,
Xenbase
Zhu,
Functional characterization of SlitPBP3 in Spodoptera litura by CRISPR/Cas9 mediated genome editing.
2016,
Pubmed
