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XB-ART-57034
Dev Cell May 21, 2020;

The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early Embryos.

Mukherjee RN , Sallé J , Dmitrieff S , Nelson KM , Oakey J , Minc N , Levy DL .


Abstract
Nuclear size plays pivotal roles in gene expression, embryo development, and disease. A central hypothesis in organisms ranging from yeast to vertebrates is that nuclear size scales to cell size. This implies that nuclei may reach steady-state sizes set by limiting cytoplasmic pools of size-regulating components. By monitoring nuclear dynamics in early sea urchin embryos, we found that nuclei undergo substantial growth in each interphase, reaching a maximal size prior to mitosis that declined steadily over the course of development. Manipulations of cytoplasmic volume through multiple chemical and physical means ruled out cell size as a major determinant of nuclear size and growth. Rather, our data suggest that the perinuclear endoplasmic reticulum, accumulated through dynein activity, serves as a limiting membrane pool that sets nuclear surface growth rate. Partitioning of this local pool at each cell division modulates nuclear growth kinetics and dictates size scaling throughout early development.

PubMed ID: 32473090
Article link: Dev Cell

GO keywords: nucleus [+]
Antibodies: Ktn1 Ab1 Nuclear Pore Complex Ab2


Article Images: [+] show captions


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