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The histone H3-H4 tetramer is a copper reductase enzyme.
Attar N
,
Campos OA
,
Vogelauer M
,
Cheng C
,
Xue Y
,
Schmollinger S
,
Salwinski L
,
Mallipeddi NV
,
Boone BA
,
Yen L
,
Yang S
,
Zikovich S
,
Dardine J
,
Carey MF
,
Merchant SS
,
Kurdistani SK
.
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Eukaryotic histone H3-H4 tetramers contain a putative copper (Cu2+) binding site at the H3-H3' dimerization interface with unknown function. The coincident emergence of eukaryotes with global oxygenation, which challenged cellular copper utilization, raised the possibility that histones may function in cellular copper homeostasis. We report that the recombinant Xenopus laevis H3-H4 tetramer is an oxidoreductase enzyme that binds Cu2+ and catalyzes its reduction to Cu1+ in vitro. Loss- and gain-of-function mutations of the putative active site residues correspondingly altered copper binding and the enzymatic activity, as well as intracellular Cu1+ abundance and copper-dependent mitochondrial respiration and Sod1 function in the yeast Saccharomyces cerevisiae The histone H3-H4 tetramer, therefore, has a role other than chromatin compaction or epigenetic regulation and generates biousable Cu1+ ions in eukaryotes.
Adamczyk,
A zinc-finger like metal binding site in the nucleosome.
2007, Pubmed
Adamczyk,
A zinc-finger like metal binding site in the nucleosome.
2007,
Pubmed
Adkins,
Chromatin disassembly mediated by the histone chaperone Asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes.
2004,
Pubmed
Agez,
Structure of the histone chaperone ASF1 bound to the histone H3 C-terminal helix and functional insights.
2007,
Pubmed
Anbar,
Oceans. Elements and evolution.
2008,
Pubmed
Bal,
Interactions of nickel(II) with histones. Stability and solution structure of complexes with CH3CO-Cys-Ala-Ile-His-NH2, a putative metal binding sequence of histone H3.
1995,
Pubmed
Brachmann,
Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.
1998,
Pubmed
Burkhead,
Elevated copper remodels hepatic RNA processing machinery in the mouse model of Wilson's disease.
2011,
Pubmed
Chen,
Structure-function studies of histone H3/H4 tetramer maintenance during transcription by chaperone Spt2.
2015,
Pubmed
Choi,
Beyond the Michaelis-Menten equation: Accurate and efficient estimation of enzyme kinetic parameters.
2017,
Pubmed
Dancis,
The Saccharomyces cerevisiae copper transport protein (Ctr1p). Biochemical characterization, regulation by copper, and physiologic role in copper uptake.
1994,
Pubmed
Davey,
Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 a resolution.
2002,
Pubmed
,
Xenbase
Dyer,
Reconstitution of nucleosome core particles from recombinant histones and DNA.
2004,
Pubmed
Forbes,
COSMIC: exploring the world's knowledge of somatic mutations in human cancer.
2015,
Pubmed
Freedman,
The role of glutathione in copper metabolism and toxicity.
1989,
Pubmed
Furukawa,
Oxygen-induced maturation of SOD1: a key role for disulfide formation by the copper chaperone CCS.
2004,
Pubmed
George,
X-ray absorption studies of yeast copper metallothionein.
1988,
Pubmed
Goldstein,
Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.
1999,
Pubmed
Graden,
Copper-mediated repression of the activation domain in the yeast Mac1p transcription factor.
1997,
Pubmed
Gregg,
Purification of mitochondria from yeast cells.
2009,
Pubmed
Gross,
Identification of the copper regulon in Saccharomyces cerevisiae by DNA microarrays.
2000,
Pubmed
Hassett,
Evidence for Cu(II) reduction as a component of copper uptake by Saccharomyces cerevisiae.
1995,
Pubmed
Hong,
Determining thermodynamic parameters from isothermal calorimetric isotherms of the binding of macromolecules to metal cations originally chelated by a weak ligand.
2008,
Pubmed
Huang,
HistoneHits: a database for histone mutations and their phenotypes.
2009,
Pubmed
Huster,
Consequences of copper accumulation in the livers of the Atp7b-/- (Wilson disease gene) knockout mice.
2006,
Pubmed
Itoh,
Novel role of antioxidant-1 (Atox1) as a copper-dependent transcription factor involved in cell proliferation.
2008,
Pubmed
Kim,
TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions.
2013,
Pubmed
Klink,
Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A.
2000,
Pubmed
Leitch,
Activation of Cu,Zn-superoxide dismutase in the absence of oxygen and the copper chaperone CCS.
2009,
Pubmed
Lin,
Suppression of oxidative damage by Saccharomyces cerevisiae ATX2, which encodes a manganese-trafficking protein that localizes to Golgi-like vesicles.
1996,
Pubmed
Liu,
Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
2014,
Pubmed
Longtine,
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae.
1998,
Pubmed
Lucas,
Electron transfer pathways in cytochrome c oxidase.
2011,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase
Mann,
Histone H3 N-terminal mutations allow hyperactivation of the yeast GAL1 gene in vivo.
1992,
Pubmed
Mattiroli,
Structure of histone-based chromatin in Archaea.
2017,
Pubmed
McRae,
Subcellular redistribution and mitotic inheritance of transition metals in proliferating mouse fibroblast cells.
2013,
Pubmed
Mesu,
New insights into the coordination chemistry and molecular structure of copper(II) histidine complexes in aqueous solutions.
2006,
Pubmed
Nevitt,
Charting the travels of copper in eukaryotes from yeast to mammals.
2012,
Pubmed
Pufahl,
Metal ion chaperone function of the soluble Cu(I) receptor Atx1.
1997,
Pubmed
Rae,
Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase.
1999,
Pubmed
Ramachandran,
Thermodynamic stability of histone H3 is a necessary but not sufficient driving force for its evolutionary conservation.
2011,
Pubmed
Rando,
Genome-wide mapping of nucleosomes in yeast.
2010,
Pubmed
Ryan,
Selection of chromosomal DNA libraries using a multiplex CRISPR system.
2014,
Pubmed
Saavedra,
Histones and metal-binding domains.
1986,
Pubmed
Sandman,
Origin of the eukaryotic nucleus.
1998,
Pubmed
Schmitt,
A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae.
1990,
Pubmed
Solomon,
Electronic absorption spectroscopy of copper proteins.
1993,
Pubmed
Spinazzi,
Assessment of mitochondrial respiratory chain enzymatic activities on tissues and cultured cells.
2012,
Pubmed
Storici,
The delitto perfetto approach to in vivo site-directed mutagenesis and chromosome rearrangements with synthetic oligonucleotides in yeast.
2006,
Pubmed
Thiele,
ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene.
1988,
Pubmed
Xu,
SAMMate: a GUI tool for processing short read alignments in SAM/BAM format.
2011,
Pubmed
Yang,
Imaging of the intracellular topography of copper with a fluorescent sensor and by synchrotron x-ray fluorescence microscopy.
2005,
Pubmed
