Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-57504
Nat Struct Mol Biol 2020 Jun 01;276:533-539. doi: 10.1038/s41594-020-0417-5.
Show Gene links Show Anatomy links

Allosteric regulation of mammalian Na+/I- symporter activity by perchlorate.

Llorente-Esteban A , Manville RW , Reyna-Neyra A , Abbott GW , Amzel LM , Carrasco N .


???displayArticle.abstract???
The Na+/I- symporter (NIS), the plasma membrane protein that actively transports I- (stoichiometry 2Na+:1I-) in thyroid physiology and radioiodide-based thyroid cancer treatment, also transports the environmental pollutant perchlorate (stoichiometry 1Na+:1ClO4-), which competes with I- for transport. Until now, the mechanism by which NIS transports different anion substrates with different stoichiometries has remained unelucidated. We carried out transport measurements and analyzed these using a statistical thermodynamics-based equation and electrophysiological experiments to show that the different stoichiometry of ClO4- transport is due to ClO4- binding to a high-affinity non-transport allosteric site that prevents Na+ from binding to one of its two sites. Furthermore, low concentrations of ClO4- inhibit I- transport not only by competition but also, critically, by changing the stoichiometry of I- transport to 1:1, which greatly reduces the driving force. The data reveal that ClO4- pollution in drinking water is more dangerous than previously thought.

???displayArticle.pubmedLink??? 32451489
???displayArticle.pmcLink??? PMC10158964
???displayArticle.link??? Nat Struct Mol Biol
???displayArticle.grants??? [+]

Genes referenced: slc5a5
GO keywords: sodium ion transmembrane transport [+]

???displayArticle.disOnts??? thyroid cancer
References [+] :
Carrasco, Iodide transport in the thyroid gland. 1993, Pubmed