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XB-ART-57561
Nucleic Acids Res January 1, 2020; 48 (22): 12648-12659.
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Quantification of the effect of site-specific histone acetylation on chromatin transcription rate.

Wakamori M , Okabe K , Ura K , Funatsu T , Takinoue M , Umehara T .


Abstract
Eukaryotic transcription is epigenetically regulated by chromatin structure and post-translational modifications (PTMs). For example, lysine acetylation in histone H4 is correlated with activation of RNA polymerase I-, II- and III-driven transcription from chromatin templates, which requires prior chromatin remodeling. However, quantitative understanding of the contribution of particular PTM states to the sequential steps of eukaryotic transcription has been hampered partially because reconstitution of a chromatin template with designed PTMs is difficult. In this study, we reconstituted a di-nucleosome with site-specifically acetylated or unmodified histone H4, which contained two copies of the Xenopus somatic 5S rRNA gene with addition of a unique sequence detectable by hybridization-assisted fluorescence correlation spectroscopy. Using a Xenopus oocyte nuclear extract, we analyzed the time course of accumulation of nascent 5S rRNA-derived transcripts generated on chromatin templates in vitro. Our mathematically described kinetic model and fitting analysis revealed that tetra-acetylation of histone H4 at K5/K8/K12/K16 increases the rate of transcriptionally competent chromatin formation ∼3-fold in comparison with the absence of acetylation. We provide a kinetic model for quantitative evaluation of the contribution of epigenetic modifications to chromatin transcription.

PubMed ID: 33238306
PMC ID: PMC7736822
Article link: Nucleic Acids Res


Species referenced: Xenopus laevis
Genes referenced: fos usp9x
GO keywords: histone acetylation [+]


Article Images: [+] show captions
References [+] :
ALLFREY, ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS. 1996, Pubmed