Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Anesth Pain Med (Seoul)
2020 Jul 31;153:291-296. doi: 10.17085/apm.20004.
Show Gene links
Show Anatomy links
Effects of tranexamic acid on the activity of glutamate transporter EAAT3.
Shin HJ
,
Lee SY
,
Na HS
,
Koo BW
,
Ryu JH
,
Do SH
.
???displayArticle.abstract???
Background: Tranexamic acid (TXA) is the most widely used hemostatic agent in surgical patients. However, when used in a high dose, it could cause a seizure in the postoperative period. The exact effector mechanism behind the seizure triggering remains unknown. Therefore, the authors investigated the effects of TXA on the activity of glutamate transporter type 3 (excitatory amino acid transporter 3; EAAT3), which is the main neuronal glutamate transporter type.
Methods: EAAT3 was expressed in Xenopus laevis oocytes through mRNA injection. Oocytes were incubated with diluted tranexamic acid for 72 h. Two-electrode voltage clamping was used to measure membrane currents before, during, and after applying 30 µM L-glutamate. Responses were quantified by integrating the current traces and reported in microcoulombs (µC). Results were presented as mean ± SEM.
Results: TXA (30 to 1,000 µM) significantly decreased EAAT3 activity. Our kinetic study showed that Vmax was significantly decreased in the TXA group compared with the control group (1.1 ± 0.1 vs. 1.4 ± 0.1 µC, n = 18-23, P = 0.043), but the Km did not significantly change (12.7 ± 3.9 µM for TXA vs. 12.8 ± 3.8 for control, n = 18-23, P = 0.986).
Conclusions: Our results suggest that TXA attenuates EAAT3 activity, which may explain its proconvulsant effect.
Beart,
Transporters for L-glutamate: an update on their molecular pharmacology and pathological involvement.
2007, Pubmed
Beart,
Transporters for L-glutamate: an update on their molecular pharmacology and pathological involvement.
2007,
Pubmed
Danbolt,
Glutamate uptake.
2001,
Pubmed
Do,
Effects of volatile anesthetics on glutamate transporter, excitatory amino acid transporter type 3: the role of protein kinase C.
2002,
Pubmed
,
Xenbase
Furtmüller,
Tranexamic acid, a widely used antifibrinolytic agent, causes convulsions by a gamma-aminobutyric acid(A) receptor antagonistic effect.
2002,
Pubmed
Guo,
Different dose regimes and administration methods of tranexamic acid in cardiac surgery: a meta-analysis of randomized trials.
2019,
Pubmed
Kalavrouziotis,
High-dose tranexamic acid is an independent predictor of early seizure after cardiopulmonary bypass.
2012,
Pubmed
Kim,
Effects of chronic exposure to ethanol on glutamate transporter EAAT3 expressed in Xenopus oocytes: evidence for protein kinase C involvement.
2005,
Pubmed
,
Xenbase
Kim,
Effects of ethanol on the rat glutamate excitatory amino acid transporter type 3 expressed in Xenopus oocytes: role of protein kinase C and phosphatidylinositol 3-kinase.
2003,
Pubmed
,
Xenbase
Kratzer,
Tranexamic acid impairs γ-aminobutyric acid receptor type A-mediated synaptic transmission in the murine amygdala: a potential mechanism for drug-induced seizures?
2014,
Pubmed
Lecker,
Tranexamic acid concentrations associated with human seizures inhibit glycine receptors.
2012,
Pubmed
Lecker,
Tranexamic acid-associated seizures: Causes and treatment.
2016,
Pubmed
Manji,
Seizures following cardiac surgery: the impact of tranexamic acid and other risk factors.
2012,
Pubmed
Maragakis,
Glutamate transporters in neurologic disease.
2001,
Pubmed
Mathews,
Neuronal glutamate uptake Contributes to GABA synthesis and inhibitory synaptic strength.
2003,
Pubmed
Merriman,
Postoperative seizure in a neurosurgical patient: Should tranexamic acid be on the differential?
2013,
Pubmed
Na,
17β-Estradiol attenuates the activity of the glutamate transporter type 3 expressed in Xenopus oocytes.
2012,
Pubmed
,
Xenbase
Sepkuty,
A neuronal glutamate transporter contributes to neurotransmitter GABA synthesis and epilepsy.
2002,
Pubmed
Sheldon,
The role of glutamate transporters in neurodegenerative diseases and potential opportunities for intervention.
2007,
Pubmed
Shin,
Caffeine-induced inhibition of the activity of glutamate transporter type 3 expressed in Xenopus oocytes.
2013,
Pubmed
,
Xenbase
Yoon,
Nicotine decreases the activity of glutamate transporter type 3.
2014,
Pubmed
,
Xenbase
Yun,
Effects of intravenous anesthetics on the activity of glutamate transporter EAAT3 expressed in Xenopus oocytes: evidence for protein kinase C involvement.
2006,
Pubmed
,
Xenbase
Zhang,
The safety and efficiency of intravenous administration of tranexamic acid in coronary artery bypass grafting (CABG): a meta-analysis of 28 randomized controlled trials.
2019,
Pubmed
