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XB-ART-57664
Dev Dyn May 1, 2021; 250 (5): 717-731.

A temporally resolved transcriptome for developing "Keller" explants of the Xenopus laevis dorsal marginal zone.

Kakebeen AD , Huebner RJ , Shindo A , Kwon K , Kwon T , Wills AE , Wallingford JB .


Abstract
BACKGROUND: Explanted tissues from vertebrate embryos reliably develop in culture and have provided essential paradigms for understanding embryogenesis, from early embryological investigations of induction, to the extensive study of Xenopus animal caps, to the current studies of mammalian gastruloids. Cultured explants of the Xenopus dorsal marginal zone ("Keller" explants) serve as a central paradigm for studies of convergent extension cell movements, yet we know little about the global patterns of gene expression in these explants. RESULTS: In an effort to more thoroughly develop this important model system, we provide here a time-resolved bulk transcriptome for developing Keller explants. CONCLUSIONS: The dataset reported here provides a useful resource for those using Keller explants for studies of morphogenesis and provide genome-scale insights into the temporal patterns of gene expression in an important tissue when explanted and grown in culture.

PubMed ID: 33368695
PMC ID: PMC8231308
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: aass acad10 acat2 ag1 ascl2 baiap2 bambi chrd.1 crb2 cul4a cyp26c1 dis3l2 efr3a ezr fmn2 foxb1 foxg1 fst fyn gpr65 gsc hes1 hoxd10 inpp5b kif14 klhl40 lrig3 myf5 myod1 nkx2-2 nkx6-2 nodal nog not nr2f2 ntn1 ogdh otx1 otx2 pax2 pax3 pax6 pbx2 pik3r4 pip4k2b prkaca ptch1 rab14 rarg rnps1 sds shh smo snai1 socs2 szl tat tbxt tmem150b tnnt3 trim33 tubb2b twist1 ventx2.1 ventx2.2 wnt4 zeb1 zic5

GEO Series: GSE160777: Xenbase,  NCBI

Article Images: [+] show captions