Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2003 Feb 17;224:776-85. doi: 10.1093/emboj/cdg080.
Show Gene links
Show Anatomy links
Homo- and heterotetrameric architecture of the epithelial Ca2+ channels TRPV5 and TRPV6.
Hoenderop JG
,
Voets T
,
Hoefs S
,
Weidema F
,
Prenen J
,
Nilius B
,
Bindels RJ
.
???displayArticle.abstract???
The molecular assembly of the epithelial Ca(2+) channels (TRPV5 and TRPV6) was investigated to determine the subunit stoichiometry and composition. Immunoblot analysis of Xenopus laevis oocytes expressing TRPV5 and TRPV6 revealed two specific bands of 75 and 85-100 kDa, corresponding to the core and glycosylated proteins, respectively, for each channel. Subsequently, membranes of these oocytes were sedimented on sucrose gradients. Immuno blotting revealed that TRPV5 and TRPV6 complexes migrate with a mol. wt of 400 kDa, in line with a tetrameric structure. The tetrameric stoichiometry was confirmed in an electrophysiological analysis of HEK293 cells co-expressing concatemeric channels together with a TRPV5 pore mutant that reduced Cd(2+) sensitivity and voltage-dependent gating. Immuno precipitations using membrane fractions from oocytes co-expressing TRPV5 and TRPV6 demonstrated that both channels can form heteromeric complexes. Expression of all possible heterotetrameric TRPV5/6 complexes in HEK293 cells resulted in Ca(2+) channels that varied with respect to Ca(2+)-dependent inactivation, Ba(2+) selectivity and pharmacological block. Thus, Ca(2+)-transporting epithelia co-expressing TRPV5 and TRPV6 can generate a pleiotropic set of functional heterotetrameric channels with different Ca(2+) transport kinetics.
Brown,
Differential effects of 19-nor-1,25-dihydroxyvitamin D(2) and 1,25-dihydroxyvitamin D(3) on intestinal calcium and phosphate transport.
2002, Pubmed
Brown,
Differential effects of 19-nor-1,25-dihydroxyvitamin D(2) and 1,25-dihydroxyvitamin D(3) on intestinal calcium and phosphate transport.
2002,
Pubmed
Bähner,
The visual G protein of fly photoreceptors interacts with the PDZ domain assembled INAD signaling complex via direct binding of activated Galpha(q) to phospholipase cbeta.
2000,
Pubmed
Bödding,
The recombinant human TRPV6 channel functions as Ca2+ sensor in human embryonic kidney and rat basophilic leukemia cells.
2002,
Pubmed
Clapham,
The TRP ion channel family.
2001,
Pubmed
Firsov,
The heterotetrameric architecture of the epithelial sodium channel (ENaC).
1998,
Pubmed
,
Xenbase
Hess,
Mechanism of ion permeation through calcium channels.
,
Pubmed
Hoenderop,
Localization of the epithelial Ca(2+) channel in rabbit kidney and intestine.
2000,
Pubmed
Hoenderop,
Calcitriol controls the epithelial calcium channel in kidney.
2001,
Pubmed
Hoenderop,
Function and expression of the epithelial Ca(2+) channel family: comparison of mammalian ECaC1 and 2.
2001,
Pubmed
Hoenderop,
Molecular mechanism of active Ca2+ reabsorption in the distal nephron.
2002,
Pubmed
Hoenderop,
Modulation of renal Ca2+ transport protein genes by dietary Ca2+ and 1,25-dihydroxyvitamin D3 in 25-hydroxyvitamin D3-1alpha-hydroxylase knockout mice.
2002,
Pubmed
Hoenderop,
Molecular identification of the apical Ca2+ channel in 1, 25-dihydroxyvitamin D3-responsive epithelia.
1999,
Pubmed
,
Xenbase
Hoenderop,
Atrial natriuretic peptide-stimulated Ca2+ reabsorption in rabbit kidney requires membrane-targeted, cGMP-dependent protein kinase type II.
1999,
Pubmed
Hofmann,
Subunit composition of mammalian transient receptor potential channels in living cells.
2002,
Pubmed
Jung,
Molecular structure of the water channel through aquaporin CHIP. The hourglass model.
1994,
Pubmed
,
Xenbase
Kamsteeg,
An impaired routing of wild-type aquaporin-2 after tetramerization with an aquaporin-2 mutant explains dominant nephrogenic diabetes insipidus.
1999,
Pubmed
,
Xenbase
Kedei,
Analysis of the native quaternary structure of vanilloid receptor 1.
2001,
Pubmed
Khanna,
Glycosylation increases potassium channel stability and surface expression in mammalian cells.
2001,
Pubmed
,
Xenbase
Kreusch,
Crystal structure of the tetramerization domain of the Shaker potassium channel.
1998,
Pubmed
Li,
TRP and the PDZ protein, INAD, form the core complex required for retention of the signalplex in Drosophila photoreceptor cells.
2000,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Lintschinger,
Coassembly of Trp1 and Trp3 proteins generates diacylglycerol- and Ca2+-sensitive cation channels.
2000,
Pubmed
Liu,
Subunit stoichiometry of cyclic nucleotide-gated channels and effects of subunit order on channel function.
1996,
Pubmed
Loffing,
Distribution of transcellular calcium and sodium transport pathways along mouse distal nephron.
2001,
Pubmed
Montell,
A unified nomenclature for the superfamily of TRP cation channels.
2002,
Pubmed
Müller,
Molecular cloning, tissue distribution, and chromosomal mapping of the human epithelial Ca2+ channel (ECAC1).
2000,
Pubmed
Müller,
Gene structure and chromosomal mapping of human epithelial calcium channel.
2000,
Pubmed
Nilius,
The single pore residue Asp542 determines Ca2+ permeation and Mg2+ block of the epithelial Ca2+ channel.
2001,
Pubmed
Nilius,
Modulation of the epithelial calcium channel, ECaC, by intracellular Ca2+.
2001,
Pubmed
Nilius,
Pharmacological modulation of monovalent cation currents through the epithelial Ca2+ channel ECaC1.
2001,
Pubmed
Peng,
Molecular cloning and characterization of a channel-like transporter mediating intestinal calcium absorption.
1999,
Pubmed
,
Xenbase
Peng,
Human calcium transport protein CaT1.
2000,
Pubmed
,
Xenbase
Strübing,
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
2001,
Pubmed
Van Abel,
1,25-dihydroxyvitamin D(3)-independent stimulatory effect of estrogen on the expression of ECaC1 in the kidney.
2002,
Pubmed
Van Cromphaut,
Duodenal calcium absorption in vitamin D receptor-knockout mice: functional and molecular aspects.
2001,
Pubmed
Vennekens,
Current understanding of mammalian TRP homologues.
2002,
Pubmed
Vennekens,
Permeation and gating properties of the novel epithelial Ca(2+) channel.
2000,
Pubmed
Voets,
CaT1 and the calcium release-activated calcium channel manifest distinct pore properties.
2001,
Pubmed
Weber,
Gene structure and regulation of the murine epithelial calcium channels ECaC1 and 2.
2001,
Pubmed
Wood,
1,25-Dihydroxyvitamin D3 increases the expression of the CaT1 epithelial calcium channel in the Caco-2 human intestinal cell line.
2001,
Pubmed
Yue,
CaT1 manifests the pore properties of the calcium-release-activated calcium channel.
2001,
Pubmed
