XB-ART-57882
Int J Mol Sci
2020 Nov 11;2122:. doi: 10.3390/ijms21228489.
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Dissection of P2X4 and P2X7 Receptor Current Components in BV-2 Microglia.
Trang M
,
Schmalzing G
,
Müller CE
,
Markwardt F
.
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Microglia cells represent the immune system of the central nervous system. They become activated by ATP released from damaged and inflamed tissue via purinergic receptors. Ionotropic purinergic P2X4 and P2X7 receptors have been shown to be involved in neurological inflammation and pain sensation. Whether the two receptors assemble exclusively as homotrimers or also as heterotrimers is still a matter of debate. We investigated the expression of P2X receptors in BV-2 microglia cells applying the whole-cell voltage-clamp technique. We dissected P2X4 and P2X7 receptor-mediated current components by using specific P2X4 and P2X7 receptor blockers and by their characteristic current kinetics. We found that P2X4 and P2X7 receptors are activated independently from each other, indicating that P2X4/P2X7 heteromers are not of functional significance in these cells. The pro-inflammatory mediators lipopolysaccharide and interferon γ, if applied in combination, upregulated P2X4, but not P2X7 receptor-dependent current components also arguing against phenotypically relevant heteromerization of P2X4 and P2X7 receptor subunits.
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Schm 536/9-2 Deutsche Forschungsgemeinschaft, MA 1581/15-2 Deutsche Forschungsgemeinschaft
Species referenced: Xenopus laevis
Genes referenced: p2rx4 p2rx7
GO keywords: microglial cell activation
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Figure 3. Effect of PSB-15417 on P2X7R-dependent ion currents. Currents were measured in HEK cells expressing hP2X7 (A) and in Xenopus oocytes expressing mP2X7 (B). Means ± SEM of 5–12 cells. Significant differences from the control are marked by asterisks. |
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