XB-ART-57898
Bio Protoc
2020 Nov 05;1021:e3802. doi: 10.21769/BioProtoc.3802.
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K+ Release Assay and K+ Measurement in Oocyte Assay.
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The Xenopus oocyte is a powerful system for the exogenous expression and functional characterization of plant membrane transport proteins. Until now, a number of potassium transporters and channels have been identified in oocytes expression system by the two-electrode voltage clamp technology. It is difficult to characterize K+/H+ anti-transporters, especially, electroneutral transporter. The K+ efflux assay system enables easy, fast, large-scale measurement of the transporters activity without two-electrode voltage clamp technology. This protocol describes a technique to measure the efflux activity of potassium transporter in oocytes expressing system.
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Species referenced: Xenopus laevis
References [+] :
Cao,
Expression of an outward-rectifying potassium channel from maize mRNA and complementary RNA in Xenopus oocytes.
1992, Pubmed,
Xenbase